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Enzyme kinetics

Total questions: 22

Worksheet time: 8mins

Name
Class
Date
1.

A biological catalyst

a)

Enzyme

b)

Coenzyme

c)

Apoenyme

d)

Holoenzyme

2.

Non protein component of an enzyme

a)

Coenzyme

b)

Cofactor

c)

Holoenzyme

d)

Apoenzyme

3.

In to how many groups enzymes are classified as

a)

Four

b)

Six

c)

Five

d)

Eight

4.

Region on enzyme where catalytic reaction occurs

a)

Catalytic site

b)

Active site

c)

Binding site

d)

Interaction site

5.

A common measure of activity is

a)

Permit number

b)

Turnover number

c)

Activity number

d)

Enzyme number

6.

Tight binding interaction of enzyme substrate indicates ......... Km value

a)

Large

b)

Initially large and then small

c)

Initially small and the large

d)

Small

7.

Weak binding interaction of enzyme and substrates indicates ..........Km value

a)

Small

b)

Initially slow

c)

Initially fast

d)

Large

8.

Which type of inhibition has structural resembles of I and E

a)

Non competitive

b)

Competitive

c)

Uncompetitive

d)

Non of the above

9.

In which type of Inhibition inhibitor binds with ES complex

a)

Uncompetitive

b)

Non competitive

c)

Competitive

d)

All of the above

10.

In which type of inhibition inhibitor has the tendency to bind with both E and ES complex

a)

Competitive

b)

Non competitive

c)

Un competitive

d)

Mixed

11.

In competitive inhibition, from lineweaver burk plot Km value

a)

Remains constant

b)

Changes

c)

Changes with addition of inhibitor

d)

None of the above

12.

In which type of inhibition both Km and Vmax changes?

a)

Competitive

b)

Non competitive

c)

Mixed inhibition

d)

Un competitive

13.

Confinement of enzyme movement is known as

a)

Inhibition

b)

Immobilisation

c)

Grouping

d)

All the above

14.

Immobilisation technique that uses semipermeable membrane for entrapment

a)

Matrix entrapment

b)

Microencapsulation

c)

Cross linking

d)

Adsorption

15.

Insoluble support medium for confining enzyme movement is

a)

Absorbent

b)

Matrix

c)

Carrier

d)

All the above

16.

To treat metabolic disorder phenyl ketonuria which type of immobilisation technique is adopted

a)

Covalent bonding

b)

Cross-linking

c)

Micro encapsulation

d)

Matrix entrapment

17.

Sensitivity of enzyme is associated with

a)

Denaturation

b)

Steric hindrance

c)

pH level

d)

None of the above

18.

Growth of micro organism is inhibited at a pressure of

a)

400-700MPa

b)

300-600MPa

c)

300-600Pa

d)

400-700Pa

19.

Optimum pH range for effective enzyme activity is

a)

2-4

b)

5.5-7.5

c)

7-13

d)

5-6

20.

pH of pepsin is ..........

a)

2

b)

4

c)

2.5

d)

3.5

21.

Which is Michaelis Menten equation

a)

V=Vmax S/(Km+S)

b)

V=Vmax S/2(Km+S)

c)

1/V=1/Vmax+Km/Vmax S

d)

V=Vmax-KmV/S

22.

Qualitative features of Michaelis Menten Equation is

a)

The reaction rate if 1st order

b)

As substrate concentration inc reaction order in substrate dec

c)

The rate of reaction is proportional to the total amount of enzyme present

d)

All the above