WorksheetsPROTEIN STRUCTURE AND ENGINEERING
Total questions: 20
Worksheet time: 7mins
Identify the catalytic traid of chymotrypsin
His 52, Asp 112, Ser 195
His 57, Asp 103, Ser 195
His 52, Asp 102, Ser 195
His 57, Asp 102, Ser 195
Which among is a non covalent interaction?
Disulphide bond
Hydrogen bond
Peptide bond
Plycosidic bond
SCID stands for
Severe combined immuno deficiency
Severe combined immuno defect
Severe child immuno deficiency
Severe child immuno defect
Serine protease are
Protein with charged serine in its chain
Enzyme without serine
Enzyme with negetively charged serine at its active site
Enzyme with negetively charged proline at its active site
What are prions?
Rogue proteins
unfolded protein
causes "mad cow" disease
All of these
NMR used for
Deducing the structure of proteins in solution
identifying the location of proteins in a mixture
detecting the presence of proteins in solution
None of these
Which among is not an application of nutraceutical proteins?
Whipping
Gelation
Fermentation
None of these
Who predicted that Hb and Sc Hb are differed in charged amino acids
V M Ingram
O farrel
Sanger
Linus Pauling
Who invented Peptide mapping
Linus Pauling
V M Ingram
Sanger
Max Perutz
Identidy the molecular difference in between Sc Hb and Hb
Sc Hb have Valine in place of Glutamic acid at 6th position in Beta chain
Hb have Valine in place of Glutamic acid at 6th position in Beta chain
Sc Hb have Glutamic acid in place of Valine at 6th position in Beta chain
Sc Hb have Glutamic acid in place of Valine at 6th position in aplha chain
Which among is not an advantage of producing insulin in milk
High production capacity
Ease of source material collection
High capital instrument and high cost
Ease of production including scale up
MALDI is a technique used in which phase of mass spectrometry?
Detection
Initiation
Acceleration
Deflection
Which among is the technique used for characterization of proteins?
Mass spectrometry
2 D Gel electrophoresis
Protein sequencing
All of these
OKT3 is a
Monoclonal Antibody
Therapeutic enzyme
poly-clonal antibody
None of these
Which among is therapeutic enzyme?
OKT3
Papain
tPA
Kappa caseine
Name a sulphur containing amino acid
methionine
aspartic acid
glycine
none of these
2d gel electrophoresis is used in characterisation of proteins because...................
It contain IEF
It separate based on m/z value
it can separate protein above 1200dalton
it has more accuracy than peptide mapping
Among the given below, which amino acid substitution gives highest activity to modified subtilisin
Cysteine
Methionine
Alanine
Serine
Chemical name of glutathion
α-glutamyl-cysteinylglycine
γ-glutamyl-cysteinylglycine
γ-acettyl-cysteinylglycine
β-glutamyl-cysteinylglycine
Chymosin is used in
confectionary
soap induxtry
none of these
cheese industry
