WorksheetsRECOMBINANT DNA TECHNOLOGY
Total questions: 30
Worksheet time: 24mins
Which of the following is an enzymatic protein that join DNA fragment between gene of interest and plasmid?
plasmid
restriction enzyme
ligase
protein expression
What is recombinant DNA?
DNA containing genes foreign to that organism
DNA which has been impacted by epigenetics
DNA that has restriction enzymes
DNA that has been sequenced
Restriction enzymes....
Cut DNA at a specific site
Can be used to cleave gene of interest
Can be used to cut vector DNA
Do all of these things
Restriction enzymes....
Cut DNA at a specific site
Can be used to cleave gene of interest
Can be used to cut vector DNA
Do all of these things
A struture of plasmid that contain gene of interest are called?
nonrecombinant plasmid
recombinant DNA
binary
selective species
Cell that receive recombinant DNA for cloning purpose
Host cell
plasmid
DNA target
Gene of interest
To produce desired protein _____________ should be taken from any source like human, animal and plant.
recombinant DNA
Target DNA
plasmid
ligase
How does restriction enzymes cleave target DNA?
By cutting the covalent bonds in the nitrogenous base.
By cutting the hydrogen bonds between the DNA strands.
By cutting the phosphodiester bond between the deoxyribose sugar and the phosphate group.
By cutting the glycosidic bonds between the deoxyribose sugar and the phosphate group.
Which of the following are TRUE of restriction enzymes?
I. It restricts transcriptionI.
II. It is found in all eukaryotic cells.
III. It acts on palindromic sequences.
IV. It is sensitive to changes in temperature and pH.
I and II
I and III
II and IV
III and IV
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA
Which of these is the "gene of interest"?
A
B
C
D
Name the structure labelled C.
restriction enzyme
recombinant plasmid
gene therapy
sticky ends
In the screening process, clones that metabolize X-gal turn_____________.
yellow
white
red
blue
One step in recombinant DNA technology is cut and cleave the DNA and plasmid. Name the enzyme used in the process shown in the diagram.
Bam HI
Eco RI
Sma I
Nael
Which of the following characteristics about D is FALSE?
able to accept recombinant DNA through transformation
able to screen the gene product from DNA
able to maintain the structure of recombinant DNA from generation to next
able to amplify the gene product from recombinant DNA
Name the process P which involved in recombinant DNA technology.
Isolation
Screening
Transformation
Insertion
Which of the following is CORRECT about host cell?
Plasmid is an example of host cell.
Unable to receive DNA molecule through transformation.
An organism that prevents cloning vectors from multiplying.
An organism that is used to receive recombinant DNA for cloning purposes.
Which of the following is TRUE regarding screening by using antibiotics and X-gal?
I. Antibiotics will prevent the growth of bacteria without plasmid.
II Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal.
III. Bacteria cells which contain nonrecombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal.
I only.
I and II only.
II and III only.
All of the above.
Which of the important characteristics of plasmid vectors used in recombinant DNA technology below is FALSE?
able to accept foreign DNA in MCS
able to replicate freely in host cell
posses selectable genetic marker
posses DNA of interest
