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WorksheetsRecombinant DNA Technology
Total questions: 35
Worksheet time: 20mins
Recombinant DNA technology
is possible only between closely related species
uses bacteria to make copies of the desired product
uses bacteria to make copies of the desired product and splices DNAs together.
uses bacteria to make copies of the desired product, splices DNAs together, and is possible only between closely related species
Through natural mechanisms, recombination occurs during crossing over between
members of the same species.
members of different species.
homologous chromosomes
base pairs on any adjacent chromosomes
The goal of genetic engineering is to?
eliminate antibiotic resistance in bacteria
isolate disease-causing plasmids
transfer of viral genes
modify cells to correct a defect or produce a desired product
These are the main tools used in recombinant DNA technology except
host cells
cosmid
restriction endonuclease
DNA polymerase
In order for DNA molecules to undergo recombination they
they must be from the same species
their strands must separate as in replication
must be cut and spliced at specific nucleotide sequences
undergo lysis
Enzymes used to cut genes in recombinant DNA research are
ligases
restriction enzymes
transcriptases
DNA polymerases
The “natural” use of restriction enzymes by bacteria is to
integrate viral DNA
destroy viral DNA
repair “sticky ends"
copy the bacterial genes
Which of the following organisms produce restriction enzymes?
Bacteria
Yeast
Retrovirus
Bacteriophage
The restriction site recognized by EcoRI is
GGCATT
GAATTC
GCCATT
GGATCC
The restriction site recognized by BamHI is
GGCATT
GCCATT
GAATTC
GGATCC
Which of the following sequences can form a palindrome?
GGCATC
GGCCGG
GGGCCC
CCGCCC
EcoRI restriction enzyme cuts plasmid at the following sites EXCEPT
G and C bond
Specific sequences
Palindromic sequences
Specific restriction sites
Which are the following statements are NOT TRUE about restriction enzymes?
Most bacterium can produce more than one type of restriction enzyme
It cleaves DNA at a specific base sequence
It restricts the replication of foreign DNA in bacteria
It produces recombinant DNA in bacteria
The fragments of chromosomes split by restriction enzymes
have fused ends
form a circle
have specific sequences of nucleotides
have random sequences of nucleotides
Restriction enzymes that perform staggered cuts are more important for the application of recombinant DNA technology compared to restriction enzymes that perform straight cuts because
it produces sticky ends
it is more efficient to anneal
it can be used at higher temperatures
it has many restriction sites
Restriction enzymes cleave DNA by cutting the
hydrogen bond between the nitrogenous base pair
phosphodiester bond between the deoxyribose sugar and phosphate group
glycosidic bond between the deoxyribose sugar and nitrogenous base
covalent bond in the nitrogenous base
DNA molecules with complementary sticky ends associate temporarily by
ionic bonds
covalent bonds
hydrogen bonds
phosphodiester bonds
The following statements are the benefits of using restriction enzymes in recombinant DNA technology EXCEPT
Its action is specific
Can be used outside of the cell
Naturally present in most bacteria
Prevent viral infection
Which of the following enzymes are NOT used in the production of a recombinant DNA?
DNA polymerase
DNA ligase
Restriction enzymes
Restriction endonuclease
What two enzymes are needed to produce recombinant DNA?
Endonuclease, transcriptase
Endonuclease, ligase
Polymerase, ligase
Transcriptase, ligase
Which of the following enzymes joins the paired sticky ends of DNA fragments?
DNA ligase
DNA polymerase
reverse transcriptase
restriction enzymes
During ligation process to form recombinant DNA, which bond is formed?
Peptide bond
Hydrogen bond
Glycosidic bond
Phosphodiester bon
What is cloning vector?
The sticky end of a DNA fragment
The enzyme that cuts DNA into restriction fragments
A DNA probe used to locate gene of interest
An agent used to transfer DNA into a living cell
Which of the following is not a cloning vector?
Plasmid
Cosmic
YAC
Bacteriophage
An example of plasmid is
sCOS1
pYAC
pUC18
λ 2001
Plasmids are important in biotechnology because they are
A vehicle for the insertion of recombinant DNA into bacteria
Recognition sites on recombinant DNA strands
Proviruses incorporated into the host DNA
Surfaces for protein synthesis in eukaryotic recombinants
Which of the following statements are the benefits of using plasmid as a vector in recombinant DNA technology EXCEPT
Can accept foreign genes
Can replicate independently in bacteria
Has a circular shape
Can be cleaved by restriction enzymes
What is transformation in recombinant DNA technology?
Insertion of foreign DNA into a cell
Synthesis of mRNA from a DNA molecule
Semiconservative replication shown by DNA
Synthesis of DNA molecule using mRNA template
Recombinant DNA technology involves 'screening' process. What is the purpose of this 'screening' process?
Isolate recombinant DNA from bacteria
Identify bacteria with recombinant DNA
Identify cell with target DNA to be cut by the restriction enzyme
Identify bacteria with plasmid to be used as a vector
Bacteria that has successfully undergo transformation are often identified by which process?
Examining the cells with an electron microscope
Using radioactive tracers to locate plasmids
Exposing the bacteria to an antibiotic that kills the cells lacking the plasmids
Producing antibodies specific for each bacterium containing a recombinant plasmids
Bacteria containing recombinant plasmid are often identified by which process?
Examining the cells with an electron microscope
Using radioactive tracers to locate plasmids
Exposing the bacteria to an antibiotic that kills the cells lacking the plasmids
Exposing the bacteria to an antibiotic with X-gal containing medium
Why is bacteria carrying a plasmid with antibiotic resistance genes are important in cloning technique?
Kills other pathogens
Helps in screening process
Produce vaccines for commercial purposes
Protects themselves from the environmental factors
The first human hormone produced successfully by using DNA recombinant technology is
human growth hormone
insulin
adenosine deaminase
thyroxin
The following are the uses of
recombinant DNA EXCEPT
Produce sheep clone
Produce agriculturally important plants and animals
Produce plants that have bacterial genes to destroy pathogenic insects
Produce microbes that secrete enzymes to overcome the problems of oil spoilage in the sea
The following are the examples of transgenic organisms EXCEPT
Pseudomonas bacteria strain can breakdown hydrocarbons
Sheep that produce milk with human growth hormone
Rhizobium bacteria in root nodules that have / the ability to fix nitrogen
Plants that are resistant to glyphosate
