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WorksheetsRECOMBINANT DNA TECHNOLOGY
Total questions: 20
Worksheet time: 15mins
Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?
amplification
insertion
transformation
screening
Which enzyme helps fuse the foreign into the new DNA?
DNA Polymerase
DNA ligase
The cell that able to receive recombinant DNA for cloning purpose known as
vector
target DNA
plasmid
host cell
To produce desired protein _____________ should be taken from any source like human, animal and plant.
recombinant DNA
Target DNA
plasmid
ligase
Bacteria use restriction enzymes to protect themselves from:
other bacteria
plants
antibiotics
viruses
Which of the following enzymes can cut nucleic acids?
DNA polymerase
restriction endonuclease
modifying enzymes
reverse transcriptase
Which restriction enzyme listed below was isolated from E. coli bacteria?
EcoRI
HindIII
HaeII
SmaI
Which of the following is NOT a tool used in genetic engineering?
plasmid
restriction enzyme
DNA helicase
DNA ligase
What type of cut is this photo?
Blunt
sticky
During insertion step,
The recombinant plasmid is inserted into a host cell
The gene of interest is inserted into a plasmid
The plasmid is inserted into the gene of interest
From where does Taq polymerase come?
Bacteria that live in hydrothermal vents/hot springs.
Cambodia
The rain forest
What happens in the Denature step of PCR?
The DNA nucleotides are broken apart.
The base-pairing rules for DNA are reversed.
The double-stranded DNA is separated into two single strands of DNA.
At what temperature does the denaturation step of PCR occur?
95° C
50° C
72° C
At what temperature does the Extension step of PCR occur?
95°C
50-65°C
72°C
If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
2
4
8
32
