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Microbial Genetics and Biotechnology

Total questions: 14

Worksheet time: 1hrs 10mins

Name
Class
Date
1.
Which of the following characteristics is not true of a plasmid?
a)
It is a circular piece of DNA
b)
It is required for normal cell function
c)
It is found in bacteria
d)
It can be trasnferred from cell to cell
2.

Which genetic material could be transmitted naturally by all three methods (transformation, conjugation & transaction) of horizontal gene transfer?

a)

an E coli chromosome

b)

the genome of a microbe

c)

drug resistance genes

d)

a and c

3.

bacteriophages (bacterial viruses) participate in this gene transfer

a)

transformation

b)

conjugation

c)

transduction

d)

translation

4.

horizontal gene transfer by plasmid is possible by

a)

transformation

b)

transduction,

c)

conjugation,

d)

transformation & conjugation

5.

Drug-resistance genes could be transferred by which of the following mechanisms:

a)

conjugation

b)

bacteriophages

c)

Transformation

d)

a, b, and c

6.

A permanent, inheritable change in the genetic information is called

a)

mutation.

b)

translation.

c)

transcription.

d)

alteration.

7.
What is the role of operons in prokaryote gene expression?
a)
It makes the genes prokaryotes be turned on
b)
It accounts for the regulation of gene activity in response to the needs of the cells
c)
To make DNA for the gene
d)
To make the gene turn of and off
8.
Operons are found in the cells of which of the following types of organisms?
a)
prokaryotes (bacteria)
b)
eukaryotes (plant and animals)
c)
animals only
d)
viruses
9.

An enzyme produced in response to the presence of a substrate is called a(n)

a)

A) inducible enzyme.

b)

B) repressible enzyme.

c)

C) restriction enzyme.

d)

D) operator.

e)

E) promoter

10.
The "central dogma" states that genetic information flows from 
a)
Protein-->mRNA-->DNA
b)
Protein-->DNA-->mRNA
c)
mRNA-->DNA-->Protein
d)
DNA-->mRNA-->Protein
11.

1. Recombinant DNA are currently used to produce____.

a)

clothing dye, cheese, and laundry products

b)

human antibiotics and vaccines

c)

crops that taste better and stay fresh longer

d)

all of these

12.

An application of using DNA technology to help environmental scientists would be ___.

a)

use PCR to analyze DNA at a crime scene

b)

create a tobacco plant that glows in the dark

c)

clone the gene for human growth hormone to treat pituitary dwarfism

d)

Make transgenic bacteria that can be used to clean up oil spills more quickly than do the natural bacteria

13.

___ are produced when DNA from another species is inserted into the genome of an organism, which then begins to produce the protein encoded on the recombinant DNA.

a)

Vectors

b)

Transgenic microbes

c)

Plasmids

d)

Gene Guns

14.

Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?

a)

The bacteria have been grown on a petri dish containing insulin.

b)

The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.

c)

The bacteria have been genetically engineered to contain the DNA needed to produce insulin

d)

The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.