Applied to the top of sucrose gradient (of increasing conc down the tube)

Gradient provides gravitational stability

Species move through and form bands based on their sedimentation coefficients (s) → Describe zonal centrifugation:

Longer distance to travel therefore better separation

Easier to remove supernatant without disturbing pellet → Fixed angle:

Electric motor

Drive shaft

Rotor to hold tubes → The sedimenting force (mω²r) is opposed by:

Using non interacting low MW solute placed in tube in a continuously decreasing conc (gradient)

Highest conc in bottom, lowest at the top → How is a density gradient prepared?

Isopycnic we separate based on equilibrium position not sedimentation coefficient → Difference between isopycnic and zonal centrifugation:

Rate of rotation (rpm) and the distance between rotor centre and the centrifuge tube → The centrifugal force generated is proportional to:

Applied to the top of sucrose gradient (of increasing conc down the tube)

Gradient provides gravitational stability

Species move through and form bands based on their sedimentation coefficients (s) → Describe isopycnic centrifugation:

Nucleic acids → Caesium chloride gradients are good for:

Sucrose or caesium chloride → What rotor is best for zonal?

up to 25,000 rpm → High speed:

After centrifugation when sublimate settles, clear liquid

An effective way of purifying liquids containing suspensions is