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WorksheetsUnit 6: DNA Analysis
Total questions: 54
Worksheet time: 29mins
The purpose of PCR is:
cutting DNA
creating RFLPs
amplifying/copy DNA
reversing the charge of DNA
What would be the complementary strand to ACGGT?
ACGGT
TGGCA
TGCCA
GTAAC
Who invented the process of DNA fingerprinting?
Alec Jeffreys
Kary Mullis
Dennis Radar
Kevin Smith
Why do you use a new pipette tip every time you get a new sample to add into the gel well?
to avoid contaminating the other samples
to use as many tools as you can
the pipette tips disappear after being used
because it is fun
The computerized database used to store DNA profiles is:
AFIS
FDDU
CODIS
SDIS
A DNA analyst need additional copies of a DNA fragment. What tool should they use?
gel electrophoresis
PCR
restriction enzymes
extraction
What is the charge of DNA?
positive
negative
neutral
purple
Which DNA strands move the fastest through the gel when the electricity is turned on?
long strands
short strands
medium strands
they all move the same
Unique pattern of DNA fragments obtained by processing a DNA sample obtained:
PCR
gel electrophoresis
DNA profiling
DNA Fingerprinting
What is a special protein that targets a specific base sequence and cuts DNA into smaller fragments?
electrophoresis
polymerase chain reaction
restriction enzyme
Introns
RFLP requires a _________ amount of DNA to work.
small
very large
normal
microscopic
Which of the following best describes how SNP analysis would be used?
create a DNA profile for prisoners
determine the criminal risk of an individual
construct medical treatment plans
figure out ancestry
Mr. Jones was killed in a car accident. Afterwards, a former girlfriend filed a paternity suit claiming that Mr. Jones was the father of her child, and thus she was entitled to the man’s estate. Which sample of DNA would have to be from Mr. Jones if he were the child’s father?
2
3
4
5
A boy moves to another country to live with his father. Years later, the boy decides to move back to live with the woman who raised him. Immigration officials suspected that the boy was the woman’s nephew, not her son, and therefore not legally entitled to residency. Based on the DNA fragments, is it possible that she is the boy’s mother?
yes
no
cannot determine with this amount of information
Which male is the father of this child?
male 1
male 2
neither could be the father
either could be the father
Which man is the father of the child?
1
2
3
4
Identify #2.
nitrogen base
phosphate group
deoxyribose sugar
hydrogen bond
CODIS is a system used to store DNA information of whom?
convicted felons
individuals charged with violent crimes
detained non US citizens
public service workers
Which of the following is a molecule labeled with a radioactive isotope, dye, or enzyme that is used to locate a particular sequence or gene on a DNA molecule?
restriction enzyme
DNA ladder
DNA probe
EtBr
Introns make up what percent of human genome?
75%
98.5%
25%
1.5%
During PCR, the temperature is increased separate DNA strands, what is this step called?
extension
contamination
annealing
denaturation
Put the following in order from 1st step to last step.
A. Stain the gel
B. PCR
C. Load the DNA
D. Analyze
E. Add restriction enzymes
F. Electrophoresis
G. Extract DNA
G, E, B, C, F, A, D
G, C, B, E, F, A, D
D, A, F, C, B, E, G
B ,D G, F, D, A, G
During PCR, the temperature is decreased to allow primers to base pair with the complementary DNA template. This step is called:
denaturation
annealing
extension
contamination
The image shows:
results of gel electrophoresis
results of capillary electrophoresis
PCR results
extracted DNA
Short DNA segments containing repeating sequences of two to five bases are called:
RFLPs
tandem repeats
VNTRs
STRs
DNA inherited only from the mother:
nuclear
mitochondrial
maternal
junk
When you extracted the DNA from cheek cells, why do you perform lysis first?
to allow the DNA to clump faster
to remove food particles and unwanted material
to break open the membranes around the cells and release the DNA
to remove all bacteria on the surface of the cell
What is the human genome?
the DNA from all your cells
all your genes
all your junk DNA
all of your DNA
What does PCR stand for?
Plethora Copies Repeated
Particular Chain Replication
Polymerase Chain Reaction
Probably Cannot React
The three steps of PCR happens is ONE CYCLE. How many cycles typically repeated?
only 1 is needed
10-15
25-30
over 1 billion
How are buffers used in electrophoresis?
conduct electricity
remove salts
add energy for movement
dissolve excess DNA
The parents of a new baby believe they brought the wrong child home from the hospital. Gel electrophoresis was performed using DNA samples from the parents and the child. A section of the gel
electrophoresis results is shown below. Which conclusion is valid based on the gel electrophoresis results?
They have the correct child, because her genetic information is identical to that of the father.
They have the wrong child, because her genetic information does not match that of either parent.
They have the correct child, because her genetic information came from both parents.
They have the wrong child, because her genetic information matches only that of the mother.
DNA fingerprinting works because
most genes are dominant
all organisms contain DNA
the most important genes are different among most people
no two people, except identical twins, have exactly the same DNA
DNA fingerprinting relies on:
difference in patterns of genes between individuals
difference in order of genes between individuals
difference in junk DNA patterns between individuals
All of these
How did police catch the Golden State killer?
by finding his DNA in CODIS
by analyzing one of his victim's DNA in CODIS
by connecting his DNA to a third cousin who was listed in a public genealogy database
pure luck
When was the DNA fingerprinting process first developed?
1984
1990
2004
2018
Restriction enzymes are called:
molecules
cutter compounds
molecular scissors
exons
Uses DNA and GEDmatch to identify victims who have not yet been named:
Parabon Nanolabs
DNA Doe Project
CODIS
John Doe Project
The first case solved using DNA profiling:
April Tinsley
Dawn Ashworth
Lynda Mann
Golden State Killer
STRs are more ______________ than RFLPs and can even be collected from bodies with severe decomposition.
enhanced
generic
stable
biased
The person responsible for the development of PCR testing is (was):
Kary Mullis
Rosalind Franklin
James Watson
Alec Jeffreys
An exon:
codes for proteins
does not code for proteins
is used for DNA profiling
has nothing to do with DNA
Mitochondrial DNA is different from nuclear DNA because (CHOOSE TWO):
it is inherited only from the mother
it is inherited only from the father
it is in the shape of a circular loop
it is in linear strands
How similar are two human's DNA?
100% the same
99.9% the same
65% the same
no similarities between two people
Which process is shown?
gel electrophoresis
polymerase chain reaction (PCR)
restriction enzymes
karyotyping
Which process is shown in the picture?
using restriction enzymes
gel electrophoresis
polymerase chain reaction (PCR)
karyotype
Which process is shown in this picture?
polymerase chain reaction (PCR)
use of restriction enzymes
karyotyping
gel electrophoresis
