Worksheets16 AP Biology Biotechnology and viruses
Total questions: 25
Worksheet time: 25mins
What is transformation?
The use of viruses to transform or genetically
engineer cells.
The measure of how well cells are transformed in a new phenotype.
The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
Genetic engineering or transformation of mammalian cells.
Cells are made competent by;
The incubation of the bacteria at 95C
A process that uses calcium chloride, cold temperatures, and heat shocking.
A process that uses GFP
A process that uses polymerases
Which of the following statement is true concerning transformation;
Bacterial transformation involves the transfer of genetic material that results in the acquisition of a new trait that is stable but not inheritable.
There are no bacterial species that go thru the transformation process naturally.
Transformation efficiencies are a way to determine how many cells were transformed per microgram of plasmid DNA used.
The promoter is the region at the end of the gene .
Observe the results above. What gene is present in the engineered plasmid?
kanamycin resistant gene
ampicillin resistant gene
tetracycline resistant gene
No plasmid is necessary to grow on plate 3.
Why do biologists use plasmids that contain an antibiotic resistance gene in transformation experiments?
So that scientists can make antibiotics
So that scientists can create super bugs.
So that scientists can identify which cells have the rDNA plasmid.
So that scientists can kill the bacteria.
Which of the following is an enzymatic protein that join DNA fragment between gene of interest and plasmid?
plasmid
restriction enzyme
ligase
protein expression
What enzyme do retroviruses use to transcribe DNA from RNA?
Reverse Transcriptase
Transcriptase
Integrase
Helicase
What two things must a virus do once it infects a host cell?
make a copy of its genetic material, transcribe and translate the genetic material
transcribe and translate the genetic material, mutate the host genome
copy its genetic material, mutate the host genome
stimulate cellular respiration, transcribe and translate the genetic material
The pieces of DNA in the diagram have restriction sites for a particular restriction enzyme. Because of a SNP, allele two only has one restriction site. Which lane represents the banding pattern for a person who is heterozygous?
the left lane
the middle lane
the right lane
The pieces of DNA in the diagram have restriction sites for a particular restriction enzyme. Because of a SNP, allele two only has one restriction site. Which lane represents the banding pattern for a person who is homozygous for allele 2?
the left lane
the middle lane
the right lane
Bacteria that did not receive a plasmid containing an ampicillin resistance gene are put on a plate containing nutrient agar that DOES contain ampicillin. What do you expect to happen?
the bacteria will create a lawn
a few colonies will be seen
the bacteria will not grow
Bacteria that did not receive a plasmid containing an ampicillin resistance gene are put on a plate containing nutrient agar that does NOT contain ampicillin. What do you expect to happen?
The bacteria will create a lawn
A few individual colonies will be seen
The bacteria will not grow
Bacteria that received a plasmid containing an ampicillin resistance gene are put on a plate containing nutrient agar that DOES contain ampicillin. What do you expect to happen?
the bacteria will create a lawn
a few colonies will be seen
the bacteria will not grow
If you want to transform E. coli with a human gene, how should the human gene be treated before it is added to the bacterial plasmid?
add exons to the DNA
remove exons from the the DNA
add introns to the DNA
remove introns from the DNA
