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Biotechnology

Total questions: 10

Worksheet time: 11mins

Name
Class
Date
1.

What is transformation?

a)

The use of viruses to transform or genetically


engineer cells.

b)

The measure of how well cells are transformed in a new phenotype.

c)

The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

d)

Genetic engineering or transformation of mammalian cells.

2.

Examine the results for group 4. Why would this group see growth without RFP in the third plate?

a)

The bacteria cells did not transform.

b)

The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.

c)

The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.

d)

Group 4 did not observe growth in the third plate.

3.

You need to pipet 100 microliters with micropipet. What numbers need to be on the dials?

a)

100

b)

010

c)

001

d)

0010

4.

___ refers to the absence of infectious material or infection

a)

clean

b)

Asepsis

c)

contaminated

5.

__ is a set of specific practices and procedures performed t make equipment and areas free from all microorganisms and to maintain that sterility.

a)

sterile technique

b)

clean

c)

asepsis

d)

surgical asepsis

6.

a sterile object becomes non-sterile when touched by a non-sterile object.

a)

true

b)

false

7.

whenever the sterility of an object is questionable, consider it sterile.

a)

true

b)

false

8.
The physical appearance of a trait is known as the-
a)
genotype
b)
phenotype
c)
characteristics
d)
intelligence
9.

The bacterial culture tube labeled P- was the control of the experiment because it contained

a)

plasmid

b)

nothing

c)

water

d)

ice

10.

The plasmid will help the bacteria in the P+ tube grow on which agar media plates?

a)

LB

b)

LB Amp

c)

Both LB and LB amp

d)

LB amp tect