WorksheetsDNA Experiment
Total questions: 9
Worksheet time: 5mins
What plant did you use to extract DNA from?
Onion
Celery
Kiwi
Geranium
What was added to the chopped cells?
Salt,
Washing up liquid
Bleach
Pepper
What was the reason for the salt?
To denature the enzyme
To clump the DNA
Break down cell membranes
Further break down cell wall
Heat the mixture at ? for how long?
room temperature for 3 seconds
Room temperature for 15 seconds
60 degrees for 15 minutes
60 degrees for 15 seconds
What are the next two steps after heating it? In order.
Let cool on the desk and blend
Blend for 5 seconds and pour into a test tube
Blend for 3 seconds and then Use filter paper
Quickly cool on ice, then blend for 3 seconds.
The mixture is filtered using what?
Coffee filter paper, because pores are larger and is quicker.
Filter paper, because it is the only thing in the lab.
Coffee filter paper, because the pores are really small so only allows the proper material to filter.
Filter paper, because the pores are the perfect size.
Protease is added to the filtrate, what is protease used for?
Separating the filtrate
Break down the proteins to release the DNA
It is an enzyme
What is the next step after adding protease?
Trickling ethanol down the side of the test tube.
Removing the DNA strands
Trickling ice cold ethanol down the side of the test tube.
Cleaning up after the experiment.
Why must the ethanol be ice cold
Ethanol will evaporate at room temperature.
DNA likes ice cold ethanol and will dissolve in it.
Ethanol is found in an ice cold state.
DNA is insoluble in ice cold ethanol,making it visible.
