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WorksheetsChapter 6 HPLC
Total questions: 14
Worksheet time: 8mins
HPLC methods include
liquid/liquid (partition) chromatography
liquid/solid (adsorption) chromatography
ion exchange nd size exclusion chromatography
all of the option correct
I Size/weight
II Polarity
III melting point
Based on a normal chromatography, the stationary phase is polar.
TRUE
FALSE
Depends on the substance
What does 'normal phase' means?
Polarity of the mobile phase is higher than stationary phase
Both stationary and mobile phase have the same polarity
Polarity of the stationary phase is higher than mobile phase
Which one of these do not associate with the HPLC component?
UV detector
Pump
Degasser
Atomizer
Choose the wrong characteristics of the eluent that can be used in the HPLC
Must be pure
Can be used without degassing
Must complied to HPLC standard
May have constant or gradient polarity
Choose the wrong statement regarding the column.
Usually made of stainless steel material
Packing materials commonly used are silica or resin
Longer column will give longer time for the mixture to separate and thus gives better resolution
Higher column ID can provide higher separation sensitivity
Which one of these that is not the common detector in HPLC?
Infra Red
Ultraviolet light
Flourescent
Mass Spectroscopy
What can be done to optimize the separation?
Adjusting operational variables
Careful selection of the column format
Calibration of the HPLC before conducting any analysis
Selecting the optimum temperature for the separation
Which one of these that can't be detected by HPLC?
Functional groups
Molecular structure
Polar compound
Non polar compound
