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WorksheetsBLAST
Total questions: 19
Worksheet time: 13mins
You have a reasonably short, typical, double-stranded DNA sequence. Basically, how many proteins can it potentially encode?
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2
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6
Which output from a BLAST search provides an estimate of the number of false positives from a BLAST search?
E value
bit score
percent identity
percent positives
query cover
You can limit a BLAST search using any ENTREZ term. For example, you can limit the results to those containting a researcher's name.
TRUE
FALSE
The BLAST algorithm compiles a list of "words" typically of three amino acids (for a protein search). Words at or above a threshold value T are defined as:
"hits," and are used to scan a database for exact matches that may then be extended
"hits," and are used to scan a database for exact or partial matches that may then be extended
"hits," and are aligned to each other
"hits," and are reported as raw scores
BLASTP is used to search a
Nucleotide query against a nucleotide sequence database
Protein query against a translated nucleotide sequence database
Translated nucleotide query against a protein database
Protein query against a protein database
Translated nucleotide query against a translated nucleotide database
BLASTN is used to search a
Nucleotide query against a nucleotide sequence database
Protein query against a translated nucleotide sequence database
Translated nucleotide query against a protein database
Protein query against a protein database
Translated nucleotide query against a translated nucleotide database
BLASTX is used to search a
Nucleotide query against a nucleotide sequence database
Protein query against a translated nucleotide sequence database
Translated nucleotide query against a protein database
Protein query against a protein database
Translated nucleotide query against a translated nucleotide database
TBLASTN is used to search a
Nucleotide query against a nucleotide sequence database
Protein query against a translated nucleotide sequence database
Translated nucleotide query against a protein database
Protein query against a protein database
Translated nucleotide query against a translated nucleotide database
TBLASTX is used to search a
Nucleotide query against a nucleotide sequence database
Protein query against a translated nucleotide sequence database
Translated nucleotide query against a protein database
Protein query against a protein database
Translated nucleotide query against a translated nucleotide database
BLAST stands for
Basic Linear Assignment Search Tool
Basic Local Alignment Search Tool
Broad Local Alignment Search Tool
Basic Linear Alignment Search Tool
The three phases of a BLAST search are
scan/extend, compile, trace-back
scan/extend, trace-back, compile
compile, scan/extend, trace-back
compile, trace-back, scan/extend
BLAST is hosted at
EBI
DDBJ
NCBI
EMBL
Raw scores are calculated from the bit scores.
TRUE
FALSE
Bit score S' is calculated from the raw score by normalizing with the statistical variables that define a given scoring system.
TRUE
FALSE
