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WorksheetsBIO615 quiz 3
Total questions: 20
Worksheet time: 12mins
A student performed a gel electrophoresis experiment. The results are represented in the diagram
below. Compared to the fragments at the top of the gel, the fragments at the lower end are
larger, and move slower
larger, and move faster
smaller, and move faster
smaller, and move slower
How are buffers used in Electrophoresis?
Conduct electricity
Remove salts
Add extension energy
Dissolve excess DNA
Southern blot is the technique for _____________ blot
RNA
Lipid
DNA
Protein
In Southern blotting, ___________ type paper is used for blot transfer
Nitrocellulose membrane or Nylon membrane filter
Whatman filter paper
Paper towel filter paper
None
Which technique is used for visualising the hybridised DNA fragments?
Chromatography
Autoradiography
Crystallography
Centrifugation
A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?
Northern blot
Southern blot
Western blot
Eastern blot
Which of the following techniques would be most useful to study gene expression?
Northern blot
Western blot
Southern blot
Eastern blot
Which two bear species are most closely related?
Bear 1 and Bear 2
Bear 1 and Bear 3
Bear 2 and Bear 3
There’s no way to tell
A newly discovered RNA virus has been causing a pandemic. You are required to detect the virus from nasal swab. Identify the method that you have to use.
rt-PCR
PCR
qPCR
Southern Blotting
Dideoxynucleoside triphosphates (ddNTPs) are used in Sanger sequencing because
ddNTPs are fluorescent
ddNTPs are incorporated very efficiently into DNA by DNA polymerase
ddNTPs cannot be incorporated into DNA by DNA polymerase
ddNTPs prevent further DNA synthesis once they are incorporated into the sequence
