Worksheets9_Disinfection and Sterilization Methods
Total questions: 9
Worksheet time: 5mins
Autoclaves sterilize by:
using high pressure to pop open microbes
creating steam at 121 degrees C
using liquid nitrogen to freeze microbes to death
boiling solutions to kill all microbes
Which chemical did Lister use?
Mercury
Chlorine
Phenol
Alcohol
Which of these chemicals can sterilize? Choose ALL that apply.
Alcohol
Bleach
Iodine
Silver
The biological entities most difficult to inactivate by any physical or chemical method are:
prions
enveloped viruses
naked viruses
MRSA
Which of these microbes would be the easiest to kill?
Mycobacterium tuberculosis
Clostridium perfringens
Escherichia coli
Bacillus anthracis
Which of the following is most resistant to heat?
endospores of bacteria
spores of fungi
cysts of protozoa
gram-positive bacteria
Which of the following would be easiest to inactivate using detergents?
enveloped viruses
naked viruses
Gram-negative bacteria
Gram-positive bacteria
UV light and other methods that use radiation target which microbial structure/component?
DNA/chromosome
PG/cell wall
PPL/cell membrane
Proteins/Enzymes
The two most common modes of action for chemicals used to control microbes are:
denaturation of proteins
disruption of membranes
inhibition of protein synthesis
interruption of DNA or RNA synthesis
dissolving capsule carbohydrates
