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WorksheetsAgarose Gel Electrophoresis
Total questions: 10
Worksheet time: 8mins
Name
Class
Date
1.
DNA fingerprinting works because
a)
Most genes are dominant
b)
All organisms contain RNA
c)
The most important genes are different among most people
d)
No two people, except identical twins, have exactly the same DNA
2.
What is the purpose of gel electrophoresis?
a)
helps cut DNA
b)
count the genes in DNA
c)
separates DNA based on size
d)
allows for an exact replicated organism
3.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
4.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
5.
A student performed a gel electrophoresis experiment. The results are represented in the diagram
below. Compared to the fragments at the top of the gel, the fragments at the lower end are
a)
larger, and move slower
b)
larger, and move faster
c)
smaller, and move faster
d)
smaller, and move slower
6.
Once DNA has been extracted from the nucleus of a cell, it is exposed to ______ ______ that act like biochemical scissors to cut the DNA.
a)
restriction enzymes
b)
gel agarose
c)
clotting factors
d)
electric shock
7.
Gel electrophoresis enables scientists to
a)
separate DNA fragments.
b)
combine DNA fragments.
c)
count the genes in DNA.
d)
insert DNA in cells.
8.
During gel electrophoresis, DNA moves from the ___ end to the ___ end.
a)
positive, negative
b)
negative, positive
9.
How are buffers used in Electrophoresis?
a)
Conduct electricity
b)
Remove salts
c)
Add extension energy
d)
Dissolve excess DNA
10.
What type of bonds hold the two strands of DNA together?
a)
Hydrogen bonds
b)
Covalent bonds
c)
Dipole-Dipole bonds
d)
Ionic bonds
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