WorksheetsThin Layer Chromatography
Total questions: 10
Worksheet time: 50mins
TLC stands for...
Tender Love & Care
Thick Layer Chromatography
Thin Layer Chromatography
Thin Liquid Chromatography
In TLC substances are separated because of differences in_______. Tick all that apply.
polarity
molecular size
concentration
electronegativity
The distance travelled by the compound depends on how soluble it is in the chosen solvent.
TRUE
FALSE
The distance travelled by the compound depends on how well it adheres to the plate.
TRUE
FALSE
What is normally required to visualise the spots on a TLC chromatogram? Tick all that apply.
X-rays
Ultraviolet light
Infrared light
Developing agent
How to calculate Rf?
Rf = distance travelled by sample / distance travelled by solvent
Rf = distance travelled by solvent / distance travelled by sample
A compound always has the same Rf value under the same conditions:______. Tick all that apply.
temperature
solvent
saturation levels
solvent volume
When could a co-spot be used?
to make a direct comparison on a TLC plate between the tested compound and the pure substance
to improve the solubility of the tested compound in the solvent
to increase the Rf value
to decrease the Rf value
The presence of more than one spot on the TLC plate shows that____
the Rf value is higher than it should be
the solvent used was too dilute
impurities are present
too much solvent was used
TLC can be used to_____. Tick all that apply
assess the purity of substances
confirm the identity of a compound
separate a mixture of immiscible liquids
purify a substance
