WorksheetsPAPA 321.3 Formative 2
Total questions: 30
Worksheet time: 35mins
State the name of the component of a HPLC that carries the mixture compounds throughout the system
stationary phase
detector
mobile phase
column
The following is an example of a mobile phase for HPLC, EXCEPT
Water
Ethanol
Hexane
Dextromethorphan
On what basis do we choose a certain mobile phase?
Chirality
Positivity
Negativity
Polarity
If chlorpheniramine maleate has a polarity of 4.1, choose the best mobile phase for it.
chloroform
methanol
petroleum ether
isooctane
A normal phase HPLC system is where the stationary phase is ________ and mobile phase is ________
polar, non polar
non polar, polar
electronegative, positive
positive, electronegative
Choose the RT of the compound clotrimazole
1.636
2.289
4.928
3.925
Based on this chromatogram, which compound has the least amount in the mixture?
metronidazole
clindamycin
clotrimazole
blank
Choose the CORRECT definition for hydrophobic
water loving
water resisting
water absorbing
water dissolving
Based on the pic, state the name of the item
TLC plate
TLC phase
TLC gas
TLC paper
Determine the Rf value of this analysis
0.73
1.8
1.5
-1.5
The pic above is the apparatus set up for ________
HPLC
GC
Column chromatography
TLC
Based on the pic above, determine the Rf value of compound C
0.2
0.4
0.6
0.8
Choose the CORRECT statement regarding the pic
The compound with the spot that travel the farthest has similar polarity with the mobile phase
The compound with the spot that travel the farthest has similar polarity with the stationary phase
The compound with the spot that travel the shortest has similar polarity with the mobile phase
Compound B has similar polarity with the mobile phase
Choose the CORRECT purpose of HPLC column
to separate the mixture
to carry the mixture solution into the system
to detect the compounds in the sample solution
to inject the sample solution into the system
The following are detectors used for HPLC, EXCEPT
photo diode array
UV-vis
Fluorescence
x-ray
Choose the BEST definition for chromatography
It is a separation technique using the solubility rate of compounds in solvents
It is a mixing method using the solubility rate of compounds in solvents
It is a titration method using the solubility rate of compounds in solvents
It is a separation techniques using the density of the compounds
Choose the CORRECT definition of spectroscopy
The study of how light interacts with matter
The study of how light absorbs matter
The study of how light reflects matter
The study of how light emit matter
According to Beer's Law, choose the CORRECT term for unknown b
molar concentration
path length
absorbance
molar absortivity
Nabila analysed a solution using UV-vis spectrophotometer and plotted a graph according to Beer's law. Choose the best statement about the graph
As the concentration increases, the absorbance also increases
As the concentration decreases, the absorbance also decreases
As the concentration increases, the absorbance decreases
As the concentration decreases, the absorbance increases
Jasper prepared a solution and analysed it with a spectrophotometer. The graph he plotted is as the above. Does his analysis obey Beer's law?
Yes
No
The following are applications of spectrometry in pharmaceutical drugs analysis, EXCEPT
assay test
identification test
dissolution test
viscosity test
Paracetamol reference standard must have the following qualities, EXCEPT
High purity
Not hygroscopic
High stability
Insoluble in solvent
Choose the reason why sodium hydroxide is a bad reference/primary standard
It is deliquiscent (has tendency to become liquid)
It has high purity
It is easily dissolved in solvent
has a molar mass
The following are the correct storage method for reference standard EXCEPT
stored in original stoppered container
stored away from heat
stored away from light
stored at room temperature
Choose the CORRECT applications of reference standards
Assay test, impurity test, friability test
Assay test, identification test, dissolution test
Impurity test, disintegration test, melting point test
Friability test, system suitability test, assay test
The following are compulsory wear if you are to enter a chemical laboratory, EXCEPT
face mask
lab coat
covered/safety shoes
gas mask
According to GLP, a chemical laboratory must have the following
work bench
pantry
mass spectrometer
gas chromatography
According to GLP, choose the CORRECT minimum requirement for instrument maintenance
once a year
twice a year
three times a year
for times a year
According to GLP, the requirement for annual training of the basics operations of the company only applies to the floor staff and not the managers
True
False
GLP is a system for non-clinical health laboratories to ensure they comply in ________, performing, monitoring, archiving and ___________
planning, reporting
testing, planning
rectifying, procrastinating
reporting, inspecting
