WorksheetsCytoskeleton MF1
Total questions: 20
Worksheet time: 14mins
Which properties a cytoskeleton must have to perform function?
Elasticity
Rigidity
Maleability
Instability
Answering which of the following key questions is critical in understanding how cytoskeleton perform its function?
How can cytoskeleton be both rigid and unstable?
What is the difference between different types of cytoskeleton?
Why ATP binds to G-actin?
Why G-actin is 42 KDa
Which domains form the base of the G-actin monomer?
I
II
III
IV
Which of the following binds to G actin
GTP
ATP
TTP
CTP
G actin monomers assemble in the same orientation to form F-actin
True
False
What is the consequence of the way the G actin monomers assemble into F-actin on the structure of Filament?
structural polarity
both ends look the same
both ends look different
filament become nonpolar
Which of the following can be used to observe actin filament structural polarity?
Scanning electron microscope
Confocal microscope
Fluorescence microscope
Transmission electron microscope
Which of the following is the function of Microtubules
Chromosome segregation
Flagella
Contractile ring
Micro villi
The end which grows faster than the other
barbed
minus
pointed
plus
Which of the following was used by scientist to decorate F-actin?
light chain myosin
meromyosin
S1 heavy meromyosin
tropomyosin
Actin polymerisation is initiated by increasing the concentration of (a)
Which of the following is false?
Actin assembles spontaneously in the cell
Actin assembly is dependent on cation concentration
Nucleation is the rate limiting step
Actin assembly is dependent on free G-actin concentration
Which of the following is true with respect to actin filaments?
Filament grows at critical concentration
The net growth of the filament at steady state is zero
Disassembly occurs at plus end above critical concentration
Assembly occur at minus end when below critical concentration
Which of the following is true with respect to actin filaments?
Filament grows at critical concentration
The net growth of the filament at steady state is zero
Disassembly occurs at plus end above critical concentration
Assembly occur at minus end when below critical concentration
Which of the following statements are TRUE?
Actin filament assembly occurs at plasma membrane
Actin filament assembly occurs at nucleus and radiates out to plasma membrane
The plus ends are associated with plasma membrane
The minus ends are associated with plasma membrane
Turnover implies that Actin filaments are
flipped 90 deg
rotated 360 deg
destroyed by protease and re synthesized
Dynamic structure where new monomers add from one and leave from other end
F-actin turnover can be visualized experimentally using
TEM
FRAP
SEM
SPECKLE
To perform FRAP what is essential?
High concentration of fluorescently labelled G-actin
Low concentration of fluorescently labelled G-actin
Incubation for equilibrium
Micro inject and observe immediately
Live cell
To perform SPECKLE what is essential?
High concentration of fluorescently labelled G-actin
Low concentration of fluorescently labelled G-actin
Incubation for equilibrium
Micro inject and observe immediately
Live cell
What according to you is the role of ATP in Microfilaments?
