wayground logo

Free Printable Worksheets

NEW

Font size

S
M
L
XL
Worksheets

DNA manipulation tools

Total questions: 16

Worksheet time: 13mins

Name
Class
Date
1.
This cuts DNA at specific sequences
a)
Plasmid
b)
Recombinant DNA
c)
Restriction enzyme
d)
Gel electrophoresis
2.
This diagram represents which of the following? 
a)
Gel electrophoresis
b)
Gene therapy
c)
Cloning 
d)
Genetic engineering
3.
The manipulation of DNA is called
a)
Genetic engineering
b)
Karyotype
c)
Plasmid
d)
Gel electrophoresis
4.

How do restriction enzymes "know" where to cut DNA

a)

A specific sequence of nucleotides

b)

They cut randomly

c)

Distance from the centromere

5.

"Sticky-ends" are DNA strands with:

a)

single-stranded ends

b)

ends cut flat by an enzyme

c)

no way to be recombined

6.
Which is used to cut DNA molecules at specific points?
a)
gel electrophoresis
b)
PCR analysis
c)
a restriction enzyme
d)
recombinant DNA
7.
Which 2 molecules form the sides (backbone) of the DNA ladder?
a)
deoxyribose sugar and adenine
b)
deoxyribose sugar and a hydrogen bond
c)
deoxyribose sugar and the nucleus
d)
deoxyribose sugar and phosphate
8.
DNA codes for...
a)
bases
b)
proteins
c)
amino acids
d)
phosphates
9.

Enzyme that connects the DNA to the backbone (glues)

a)

Ligase

b)

Polymerase

c)

Helicase

d)

Primase

10.

DNA polymerase moves in the _________ to ____________ direction

a)

3' to 5'

b)

easiest

c)

5" to 3"

d)

9 to 5

11.

DNA strand has the following bases AAGCCA what are the bases on the complementary strand

a)

GGCTTA

b)

TTGGGA

c)

CCAGGT

d)

TTCGGT

12.

Number 1 in the picture above

a)

Replication

b)

DNA Polymerase

c)

Primase

d)

Ligase

13.
Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.
a)
polymerase
b)
ligase
c)
dehydrogenase
d)
lactase
14.

A molecule labeled with radioactive isotope, dye or enzyme that is used to locate a particular sequence or gene.

a)

DNA Probe

b)

DNA Fingerprint

c)

Electrophoresis

15.

What is the correct term for a restriction enzyme?

a)

DNA ligase

b)

Taq polymerase

c)

Endonuclease

d)

Manorlakease

16.
During replication, what enzyme adds complimentary bases?
a)
helicase
b)
synthesase
c)
ligase
d)
polymerase