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WorksheetsTranscription
Total questions: 16
Worksheet time: 9mins
Name the enzyme that facilitates opening of DNA helix during transcription.
DNA polymerase
RNA polymerase
DNA ligase
DNA helicase
Which of the following is a wrong match?
Rho factor - Terminates translation
Template strand – Has same sequence as of mRNA
Sigma factor - Initiates translation
All of these
Code in RNA corresponding to ATGC in DNA
TACG
TACA
AGUC
UACG
In the absence of which factor or enzyme, the transcription starts non-specifically (hypothetically) at any base of DNA ?
Rho ( ρ ) factor
Core enzyme
Sigma ( σ ) factor
None of the above
In initiation of transcription, there is
Attachment of RNA polymerase holoenzyme at promoter site.
Terminal addition of 200-300 adenylated nucleotide at 3' end of m-RNA
ATPase activity by rho factor resulting in release of polynucleotide chain
Binding of multiple ribosomes at single mRNA.
Which of the following sequences of RNA will be transcribed from a hypothetical sequence of DNA given below?
3'-ATGCATGCATGCATGC-5'
5'-TACGTACGTACGTACG-3'
3'-UACGUACGUACGUACG-5'
5'-AUGCAUGCAUGCAUGC-3'
5'-UACGUACGUACGUACG-3'
3'-AUGCAUGCAUGCAUGC-3'
Find the correct statement regarding eukaryotic transcription
There is single DNA dependent RNA polymerase that catalyses transcription of all types of RNA
Elongation is controlled by sigma factor
Termination factor associates with DNA polymerase enzyme during termination of transcription
RNA polymerase II catalyses the transcription of hnRNA in eukaryotes.
Mark the option depicting correct labelling for the image given below.
A- Template strand, B- Coding Strand, C- Terminator, D-Promoter
A- Structural gene, B- Template strand, C- Promoter, D- Terminator
A- Coding Strand, B- Template strand, C- Terminator, D- Promoter
A- Coding Strand, B- Structural gene, C- Promoter, D- Terminator
Promoter region is located towards the
5’ end of the coding strand of gene
3’ end of the template strand of gene
5’ end of the template strand of gene
Both (A) and (B)
If the sequence of coding strand is 5’ GCCGTACCGTTACG 3’ , then the sequence of mRNA will be
5’ GCAUUGCCAUGCCG 3’
3’ CGGUACCGUUAGCC 5’
5’ GCCGUACCGUUACG 3’
3’ CGGCAUGGCAAUGC 5’
Select the incorrectly matched pair.
α2ββ’ω– Core enzyme of RNA polymerase
ρ factor – Chain termination in translation
σ factor – Initiation factor in transcription
Promoter – A-T rich region of transcription unit
Which strand is responsible for coding the mRNA from transcription unit?
Template strand
Coding strand
Lagging strand
Leading strand
Read the following statements and select the correct statements:
Statement I: Recognition of promoter region is done by sigma (σ) factor in prokaryotes.
Statement II: Split-gene arrangement represents an ancient feature of genome.
Only statement I is correct
Only statement II is correct
Both statement I and II are correct
Both statement I and II are incorrect
The enzyme DNA dependent RNA polymerase catalyses the polymerization reaction in _________ direction.
only 5’ → 3’
only 3’ → 5’
both A. and B.
none of these
In transcription in eukaryotes, heterogenous nuclear RNA (hnRNA) is transcribed by
RNA polymerase I
RNA polymerase II
RNA polymerase III
All of these.
Trancription in prokaryotes occurs in ____________ whereas in eukaryotes it occurs in _____________.
(a)
