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Cell and Tissue Microscopy (AS Biology)

Total questions: 15

Worksheet time: 16mins

Name
Class
Date
1.
A microscope has a resolution of 200nm. Which of the following organisms would not be resolved using this microscope?
a)
Chloroplast
b)
lysosomes
c)
mitochondria
d)
ribosomes
2.

Which group of structures are visible in a suitably stained plant cell using a high power (x400) light microscope.

a)

A

b)

B

c)

C

d)

D

3.

Which size ranges can be viewed using a light microscope?

a)

4 only

b)

1 and 2 only

c)

2 and 3 only

d)

3 and 4 only

4.

The diagram shows a stage micrometer, with divisions 0.1mm apart, viewed through an eyepiece containing a graticule. What is the area of the field of view of the mciroscope at this magnification (π=3.14)

a)

π=12.5 x 12.5 = 4.9 x 102μm2

b)

π=50 x 50 = 7.9 x 103μm2

c)

π=125 x 125 = 4.9 x 104μm2

d)

π=250 x 250 = 2.0 x 105μm2

5.
An electron microscope has a higher resolution than a light microscope. Which is a result of the higher resolution?
a)
the ability to produce larger images of cells
b)
the ability to see cristae in mitochondria
c)
the ability to see mRNA in all cells
d)
the ability to see the nucleus in eukaryotes
6.
Eyepiece graticules and stage micrometers are used to measure cells. Which is the correct reason why an eyepiece graticule is calibrated?
a)
An eyepiece graticule can be used to make measurement
b)
An eyepeice graticule is magnified by the objective lens
c)
An eyepiece graticule magnifies the specimen
d)
An eyepiece graticule makes comparison
7.
What best describes an electron microscope in comparison to a light micrsocope?
a)
higher magnification and higher resolution
b)
higher magnification but lower resolution
c)
lower magnification but higher resolution
d)
lower magnification and lower resolution
8.
At approximately which magnification is light microscopy not suitable because the resolution becomes too low?
a)
x100
b)
x200
c)
x400
d)
x1500
9.

The diagram shows a stage micrometer viewed with an eyepiece graticule scale, using a magnification of x400. Using the same magnification, a chloroplast is measured and found to be 4 eyepiece graticule divisions long. How long is the chloroplast?

a)

1.0 x 101 μm

b)

4.0 x 102 μm

c)

2.5 x 10-1 μm

d)

2.5 x 10-2μm

10.
The eyepiece lens of a microscope is fitted with an eyepiece graticule. Which statement about the graticule are correct? (1) it allows you to measure the actual length of cells (2) it allows you to draw cells with correct proportions (3) it changes in size as the objective lens changes from x10 to x40
a)
1, 2 and 3
b)
1 and 3 only
c)
1 only
d)
2 only
11.

The diameter of living cells varies considerably. The diameter of a typical eukaryotic cell is 1.5 × 101 μm. The diameter of a typical prokaryotic cell is 7.5 × 102 nm. Using these measurements, what is the maximum number of each cell type which could fit along a line 1 cm long?

a)

A

b)

B

c)

C

d)

D

12.

What is the diameter of a typical plant cell?

a)

4.0 x 101 Um

b)

1.0 x 100 Um

c)

4.0 x102nm

d)

1.0 x 102 nm

13.

A specimen is viewed under a microscope using green light with a wavelength of 510 nm. If the same specimen is viewed under the same conditions, but using red light with a wavelength of 650 nm instead, what effect will this have on the magnification and on the resolution of the microscope?

a)

A

b)

B

c)

C

d)

D

14.

Ribosomes consist of two subunits, each containing rRNA. An analysis of all the 70S ribosomes from a single cell of the bacterium, Escherichia coli, showed that there were:

• 38 000 rRNA molecules

• 2 main types of rRNA molecule

• 19 000 copies of each type of rRNA molecule

How many 70S ribosomes were there in the E. coli cell?

a)

9500

b)

19000

c)

38000

d)

76000

15.

With reference to the table, which organelles will be clearly visible under the high power (x400) of the light microscope?

a)

A

b)

B

c)

C

d)

D