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WorksheetsCell and Tissue Microscopy (AS Biology)
Total questions: 15
Worksheet time: 16mins
Which group of structures are visible in a suitably stained plant cell using a high power (x400) light microscope.
A
B
C
D
Which size ranges can be viewed using a light microscope?
4 only
1 and 2 only
2 and 3 only
3 and 4 only
The diagram shows a stage micrometer, with divisions 0.1mm apart, viewed through an eyepiece containing a graticule. What is the area of the field of view of the mciroscope at this magnification (π=3.14)
π=12.5 x 12.5 = 4.9 x 102μm2
π=50 x 50 = 7.9 x 103μm2
π=125 x 125 = 4.9 x 104μm2
π=250 x 250 = 2.0 x 105μm2
The diagram shows a stage micrometer viewed with an eyepiece graticule scale, using a magnification of x400. Using the same magnification, a chloroplast is measured and found to be 4 eyepiece graticule divisions long. How long is the chloroplast?
1.0 x 101 μm
4.0 x 102 μm
2.5 x 10-1 μm
2.5 x 10-2μm
The diameter of living cells varies considerably. The diameter of a typical eukaryotic cell is 1.5 × 101 μm. The diameter of a typical prokaryotic cell is 7.5 × 102 nm. Using these measurements, what is the maximum number of each cell type which could fit along a line 1 cm long?
A
B
C
D
What is the diameter of a typical plant cell?
4.0 x 101 Um
1.0 x 100 Um
4.0 x102nm
1.0 x 102 nm
A specimen is viewed under a microscope using green light with a wavelength of 510 nm. If the same specimen is viewed under the same conditions, but using red light with a wavelength of 650 nm instead, what effect will this have on the magnification and on the resolution of the microscope?
A
B
C
D
Ribosomes consist of two subunits, each containing rRNA. An analysis of all the 70S ribosomes from a single cell of the bacterium, Escherichia coli, showed that there were:
• 38 000 rRNA molecules
• 2 main types of rRNA molecule
• 19 000 copies of each type of rRNA molecule
How many 70S ribosomes were there in the E. coli cell?
9500
19000
38000
76000
With reference to the table, which organelles will be clearly visible under the high power (x400) of the light microscope?
A
B
C
D
