NEW
Font size
WorksheetsModule 3: Microtomy
Total questions: 21
Worksheet time: 11mins
microtome most commonly used in histo lab
clinical freezing
sliding microtome
ultra microtome
rotary micromtome
microtome which the block is stationary and a knife is moved horizontally
clinical freezing
rotary
sliding
which microtome moves the block up and down ?
sliding
clinical freezing
cryostat
rotary
which microtome sections large paraffin blocks?
rotary
clinical freezing
vibrating
sliding
which microtome cuts fresh, frozen tissue
rotary
sliding
clinical freezing
which microtome clamps to the tabletop?
ultramicrotome
sliding
clinical freezing
angle formed by the intersection of the block face and adjacent cutting facet of knife.
bevel angle
clearance angle
angle that is usually 28-32 degrees
bevel angle
clearance angle
wedge angle
angle that is set to 3-8 degrees
bevel angle
clearance angle
wedge angle
angle made when 2 cutting facets intersect
bevel angle
clearance angle
wedge angle
angle between cutting facet and block of tissue.
wedge angle
clearance angle
bevel angle
angle that is set at 15 degrees
clearance angle
wedge angle
bevel angle
this blade is used for dense tissue
low profile
high profile
this blade is used for routine sectioning
high profile
low profile
what is most likely the cause for crooked ribbons?
insufficient dehydration or clearing
too little blade tilt
top and bottom edges not parallel
dull blade
what is most likely the cause for washboarding?
dull blade
too much blade tilt
too little blade tilt
block not evenly chilled
what is most likely the cause for vertical scratches in the sections?
defect in blade edge
dull blade
insufficient dehydration during processing
paraffin too soft
most likely the cause for failure of a ribbon to form.
too little blade tilt
too much blade tilt
hard tissue
overfixed tissue
how to resolve issue of section lifting from blade as block is raised?
change room temp
decrease blade tilt
increase blade tilt
replace blade
solution for block face unevenly sectioned
face less aggressively
increase blade tilt
choose paraffin with lower melting point
realign microtome
solution for holes in sections
realign microtome
ensure block is evenly chilled
increase blade tilt
face less aggressively
