Worksheetsproteomics
Total questions: 25
Worksheet time: 9mins
What is the length of a motif, in terms of amino acid residues?
30-60
10-20
70-90
1-10
Which of the following tools is used for the identification of motifs?
BLAST
PROSPECT
COPIA
Pattern hunter
Gene identification in prokaryotic organisms is simplified by their lacking __________.
Introns
Exons
Coding segments
Useful nucleotide sequence
Screening the predicted protein sequences against_________ library confirms the prediction and expression of the gene.
Expressed sequence tag(EST)
Tags
Palindrome
Proteomes
Each genome sequence is scanned for protein-encoding genes using gene models trained on known gene sequences from the same organisms.
True
False
In a living organisms, strong interactions between molecules are formed by a certain type of bond.Name that?
Hydrogen bonding
Covalent bond
Electrostatic interactions
Van der waals forces
Proteolytic enzyme trypsin cleaves protein at which of these specific sites?
Carboxyl side of Alaine, Glycine
Carboxyl side of aromatic amino acid
Amino acid side of aromatic amino acid
Carboxyl side of Lysin or Arginine
Sequencing of genomes depends on the assembly of a large number of DNA reads into a linear, contiguous DNA sequence.
True
False
Protein folding is a process in which a polypeptide folds in to________________.
2-D structure
Globular form
3-D structure
Linear form
In the zinc finger, which residues in this sequence motif form ligands to a zinc ion?
Cysteine and histidine
Cysteine and arginine
Histidine and proline
Histidine and arginine
The protein structural motif domain -helix loop helix are contained by all of the following except ________.
Scleraxis
Neurogenins
Transcription factor 4
Leucine zipper
Motifs that can form α/ horseshoes confirmation are rich with which protein residue?
Proline
Arginine
Valine
Leucine
Which of the following name is given to molecular chaperones?
Allosteric protein
Heat shock protein
Denaturation protein
Ribonuclease
Which of these heat shock proteins prevent protein misfolding and maintain polypeptide chain in the unfolded state.
a) Hsp60
b) Hsp28
c) Hsp70
d) Hsp32
Hsp60
Hsp28
Hsp70
Hsp32
Among all the heat shock proteins which one is known as chaperonins?
Hsp70
Hsp32
Hsp60
Hsp30
Which of the common structural motifs are described wrongly?
β-hairpin – adjacent antiparallel strands
Greek key – 4 adjacent antiparallel strand
β-α-β – 2 parallel strands connected by helix
β-α-β – 2 antiparallel strands connected by helix
Which of the following wrongly describes protein domains?
They are made up of one secondary structure
Defined as independently foldable units
They are stable structures as compared to motifs
They are separated by linker regions
The main issue in prediction of eukaryotic genes is the identification of exons, introns, and splicing sites.
True
False
Most vertebrate genes use __________ as the translation start codon and have a uniquely conserved flanking sequence call a Kozak sequence (CCGCCATGG).
AAG
ATG
AUG
AGG
Which of the following is true regarding the methods of gene prediction?
They solely consist of a type called ab initio–based methods
The ab initio–based approach predicts genes based on the given sequence alone
The ab initio–based approach predicts genes based on the given sequence and relative homology data
They solely consist of a type called homology-based approaches
Which of the following is not known to be involved in the process of assisted folding of proteins?
Chaperonins
Heat shock proteins
Peptide bond hydrolysis
Peptide bond isomerization
The effects of protein on an entire organism is described in __________
Phenotypic function
Cellular function
Molecular function
Structural genomics
Sequencing of genomic DNA is included in __________
Phenotypic function
Cellular function
Molecular function
Structural genomics
Genes of same species, similarly related to each other are _________.
Ortholog
Synteny
Paralog
Microarray
Genes of different species but possessing a clear sequence and functional relationship to each other are __________
Ortholog
Synteny
Paralog
Microarray
