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WorksheetsProteomics pavi
Total questions: 21
Worksheet time: 7mins
The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis.
True
False
Which of the following is incorrect regarding 2D-Page?
It stands for Two-dimensional polyacrylamide gel electrophoresis
It separates proteins by charge only
The gel is run in one direction in a pH gradient under a non-denaturing condition
It works to separate proteins by isoelectric points (pI)
Which of the following is not correct about the X-ray Crystallography?
In x-ray protein crystallography, proteins need to be grown into large crystals in which their positions are fixed in a repeated, ordered fashion
The protein crystals are illuminated with an intense x-ray beam
The x-rays are deflected by the electron clouds surrounding the atoms in the crystal producing a regular pattern of diffraction
The protein crystals are illuminated with an intense infrared beam
Which of the following is not correct about the NMR?
a)
b)
c)
d)
NMR spectroscopy detects spinning patterns of atomic nuclei in a magnetic field
It stands for Nuclear Magnetic Resonance
NMR spectroscopy detects spinning patterns of atomic nuclei in a electric field
Protein samples are labeled with radioisotopes such as 13C and 15N
The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by?
Edman
Sanger
Mendel
Watson and Crick
Which of the following is the correct order of sequencing?
Cleaving, sequencing and ordering
Sequencing, ordering and cleaving
Ordering, cleaving and sequencing
Ordering, sequencing and cleaving
Edman degradation is used for _________
Identifying N-terminal amino acids
Identifying C-terminal amino acids
Identifying amino acid
Identifying carbohydrates
What is the length of a motif, in terms of amino acids residue?
30- 60
10- 20
70- 90
50-70
In agarose gel electrophoresis, the DNA is moved towards the
cathode
anode
DNA doesn't move
Moves slowly
Proteins that do not function but recover their activity upon folding are________
Denatured
folded
synthesized
hydrolyzed
The study of the full complement of proteins expressed by a genome is called __________
Proteome
Proteomics
Genomics
Protein formation
What is the goal of structural proteomics project?
To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function
To identify and sequence of all the genes present in the human body
To introduce new genes to human beings
To remove disease causing genes from humans
Protein structure refers to
One dimension
Two dimension
Three dimension
Which type of protein are highly stable?
Primary
Secondary
Tertiary
The structure of protein can be destroyed by which rays?
Ultra violet rays
Infrared rays
Radio waves
Micro waves
The first protein sequenced by Fredrick Sanger is
Hemoglobin
Insulin
Myosin
Myogoblin
At high temperature proteins are
Denatured
Destroyed
Inactive
Active
What are all the protein analysis techniques
Protein separation
Protein identification
Western blotting
All of the above
Protein absorbs uv light at the range of
280nm
270nm
250nm
350nm
SDS disrups protein by changing the
Primary structure of protein
Secondary and tertiary structure of protein
Primary and secondary structure of protein
Secondary,tertiary and Quaternary structure of protein
Which of the following statements are correct for globular proteins?
Always contain beta sheet
contain both alpha and beta sheet
Contain beta turns
Always Contains alpha helix
