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Analysis of protein

Total questions: 17

Worksheet time: 11mins

Name
Class
Date
1.

Select the essential amino acid

a)

Lysine

b)

Valine

c)

Aspartic

d)

Glutamic Acid

2.

Which property of meat depends on protein

a)

Tenderness

b)

Colour

c)

Bloom

d)

Taste

3.

What are the three basic steps of Kjeldhal Method

(a)  

4.

In the digestion step what chemical are used

a)

Kjeldhal catalyst

b)

Sulphuric Acid

c)

Boric acid

d)

Sodium hydroxide

5.

In the distillation step, the digested sample is made alkaline with NaOH and the nitrogen is distilled off as?

a)

Ammonium ions

b)

Ammonia gas

c)

Ammonium hydroxide

d)

Ammonium acetate

6.

Ammonia is trapped in what solution??

a)

Sodium hydroxide solution

b)

Boric acid solution

c)

Sulphuric acid solution

d)

Hydrochloric acid

7.

Most commonly conversion factor used to determine crude protein from total nitrogen content is

a)

6

b)

6.25

c)

7

d)

6.5

8.

In dumas method temperature used for combustion is ?

a)

900 degree Celsius

b)

800 degree Celsius

c)

1000 degree Celsius

d)

850 degree Celsius

9.

DUMAS instrument is calibrated by analyzing a material that is pure and has a known nitrogen concentration, such as

a)

EDTA

b)

Tryptophan

c)

Lysine

d)

Ammonia

10.

Which oxide is used to convert Food components to gases in Dumas method

a)

Copper oxide

b)

Sulphur oxide

c)

Zinc oxide

d)

Carbon oxide

11.

Carbon dioxide is trapped by which chemical in Dumas method

(a)  

12.

Tryptophan and tyrosine absorb ultraviolet light strongly at what nm?

a)

280

b)

540

c)

290

d)

300

13.

Biuret reagent reacts with what to produce violet purple color?

(a)  

14.

What wavelength is used to determine protein content in biuret method

a)

540

b)

280

c)

560

d)

520

15.

Folin-Ciocalteau phenol reagent is used in what method

(a)  

16.

What Color is produced in Lowry method?

a)

Blue

b)

Violet

c)

Red

d)

Purple

17.

What is the charge on Dye in Dye binding method

a)

Negative

b)

Positive