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Chapter 6: Expression of biological information

Total questions: 32

Worksheet time: 19mins

Name
Class
Date
1.

The two molecules that alternate to form the backbone of a polynucleotide chain are:

a)

adenine and thymine.

b)

cytosine and guanine.

c)

sugar and phosphate.

d)

base and sugar

2.

What kind of chemical bond is found between paired bases of the DNA double helix?

a)

Hydrogen

b)

Ionic

c)

Covalent

d)

Phosphate

3.

Which of the following represents a similarity between RNA and DNA?

a)

Bothe are double-stranded and found exclusively in the nucleus.

b)

The presence of uracil.

c)

The presence of an OH group on the 2’ carbon of the sugar.

d)

Nucleotides consisting of a phosphate, sugar and nitrogenous base.

4.

The strands that make up DNA are antiparallel. This means that...

a)

the twisting nature of DNA creates nonparallel strands.

b)

the 5’ to 3’ direction of one strand runs counter to the 5’ to 3’ direction of the other strand.

c)

base pairings create unequal spacing between the two DNA strands.

d)

one strand is positively charged and the other is negatively charged.

5.

What kind of molecule or substance is the primer that is used to initiate the synthesis of a new DNA strand?

a)

RNA

b)

DNA

c)

Protein

d)

phosphate

6.

Primase is the enzyme responsible for:

a)

unwinding the DNA double strand to allow DNA polymerase access to the template DNA

b)

introducing nicks into the DNA double strand in order to prevent the formation of knots.

c)

hydrolyzing ATP to facilitate DNA unwinding.

d)

making short strands of RNA at the site of replication initiation.

7.

RNA synthesis is also known as:

a)

elongation.

b)

reverse transcription.

c)

translation.

d)

transcription.

8.

A particular triplet of bases in the template strand of DNA is AGT. The corresponding codon for the mRNA transcribed is

a)

AGT

b)

UGA

c)

TCA

d)

ACU

9.

Translation occurs in..

a)

the cytoplasm.

b)

a lysosome.

c)

the nucleus.

d)

a ribosome.

10.

All of the following are directly involved in translation except

a)

mRNA

b)

tRNA

c)

ribosomes

d)

DNA

11.

In all organisms, the AUG codon codes for:

a)

the initiation of translation.

b)

the termination of transcription

c)

the termination of chain elongation.

d)

the amino acid valine.

12.

The anticodon of a particular tRNA molecule is...

a)

complementary to the corresponding mRNA codon.

b)

complementary to the corresponding triplet in rRNA

c)

the part of tRNA that bonds to a specific amino acid.

d)

changeable, depending on the amino acid that attaches to the tRNA.

13.

A particular triplet of bases in the coding sequence of DNA is AAA. The anticodon on the tRNA that binds the mRNA codon is...

a)

TTT

b)

UUA

c)

UUU

d)

AAA

14.

What are ribosomes composed of?

a)

rRNA only

b)

proteins only

c)

both rRNA and protein

d)

mRNA, tRNA, rRNA and protein

15.

Which of the following components are present upon the completion of translation initiation?

a)

Small ribosomal subunit.

b)

Large ribosomal subunit.

c)

Initiator tRNA.

d)

All of the above.

16.

A polyribosome is...

a)

a complex of many ribosome and an mRNA.

b)

a complex of many ribosomes in eukaryotes.

c)

an initiation complex in eukaryotes.

d)

an elongation complex in eukaryotes

17.

A new DNA strand only elongated in the 5’ to 3’ direction because

a)

DNA polymerase adds nucleotides at the 5’ end

b)

Replication must progress toward the replication fork

c)

Okazaki fragments prevent elongation in the 3’ to 5’ direction

d)

DNA polymerase adds nucleotides only to the free 3’ end

18.

DNA ligase are enzymes that

a)

Unwind the double helix of DNA during replication

b)

Link the nucleotides of the new DNA strand along the template of the old strand

c)

Rejoin the two DNA strands (one new and one old) after replication

d)

Seal together the broken ends of DNA strands

19.

Replication of DNA produces

a)

RNA molecule

b)

Only new DNA

c)

Two DNA molecule

d)

Excessive DNA

20.

What is the function of DNA strand during DNA replication?

a)

Replicate

b)

Substitute into new strand

c)

Template

d)

Complementary

21.

Which of the following does NOT involved in transcription?

a)

Stop codon

b)

RNA polymerase

c)

Terminator

d)

Promoter

22.

Which of the following is NOT considered as a part of a lactose operon?

a)

Regulatory gene

b)

Promoter

c)

Operator

d)

Structural genes

23.

Which of the following is the major enzyme that involved in DNA

replication?

I. Helicase

II. Reverse transcriptase

III. Topoisomerase

IV. DNA polymerase

V. Aminoacyl-tRNA synthetase

a)

I, III and IV

b)

I, III and V

c)

I,II, III and IV

d)

II, III, IV and V

24.

Which of the following start the synthesis of a new DNA?

a)

RNA primer

b)

DNA polymerase

c)

Okazaki fragment

d)

DNA ligase

25.

What is the role of DNA ligase in DNA replication?

a)

Synthesize of short RNA primer by adding RNA nucleotide

b)

Replaces the RNA primer by adding the DNA nucleotide

c)

Join Okazaki fragment at lagging strand forming phosphodiester bond

d)

Unwind the double stranded DNA to become single stranded DNA

26.

In DNA

replication, new nucleotides are added to a growing DNA by…………………

a)

DNA helicase

b)

DNA polymerase III

c)

RNA polymerase

d)

RNA primer

27.
Replicate the following strand of DNA: 
AATCATGGA
a)
AATCATGGA
b)
TTAGTACCT
c)
UUAGUACCU
d)
GGATAUCUA
28.
The enzyme that unzips the DNA to prepare for replication
a)
helicase
b)
replicase
c)
polymerase
d)
synthase
29.
Which phase of which process does this picture show?
a)
Transcription
b)
Translation
c)
Replication
30.
A process in which DNA has the unique ability to make an exact copy of itself.
a)
Replication
b)
Translation
c)
Transcription
31.
Which enzyme is responsible for adding nucleotides?
a)
Topoisomerase
b)
DNA Polymerase
c)
Ligase
d)
Primase
32.
The process of DNA replication is called semi conservative because it results in
a)
none of these
b)
Two DNA molecules.  One original molecule and one with two new strands
c)
two exact copies with new strands
d)
two identical molecules, each with 1 original strand and one new.