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Translational regulation & protein folding

Total questions: 14

Worksheet time: 8mins

Name
Class
Date
1.

Which of these are global mechanisms to regulate translation?

a)

Non-sense Mediated Decay

b)

Codon usage

c)

phosphorylation of eIF2 & eIF2B

d)

mRNA stability

e)

phosphorylation of 4E-BPs

2.

Secondary structures located at the 5' UTR

a)

enhance translation

b)

slow down translation

c)

have no effect on translation

3.

IRP is an example of a

a)

Carrier protein

b)

Repressor protein

c)

Enhancer protein

4.

miRNA and the RISC regulate translation at what stage

a)

elongation stage

b)

initiation stage

c)

termination stage

5.

Which triggers faster degradation?

a)

siRNA

b)

miRNA

6.

Phosphorylation of eIF2 & eIF2B result in

a)

negative regulation

b)

positive regulation

7.

Which kinase phosphorylates 4E-BPs?

a)

PKR (protein kinase R)

b)

mTOR

c)

eIF4E

8.

Protein disulfide isomerase is found in the

a)

nucleus

b)

ER lumen

c)

mitochondria

d)

lysosomes

e)

cytoplasm

9.

------ have complete complementarity while --------- have mismatches.

a)

miRNA, siRNA

b)

siRNA, miRNA

c)

RISC, RITS

d)

RITS, RISC

10.

--------- sequences, allow viruses to bypass the cap-dependent mechanism of translation

a)

IRP

b)

IRE

c)

IRES

d)

ORF

11.

What dictates the final folded structure of a protein is its

a)

primary structure

b)

secondary structure

c)

tertiary structure

d)

quaternary structure

12.

To prevent protein aggregation, ___________ bind to hydrophobic patches, and ___________ provide an isolated space for proteins to fold properly.

a)

chaperonins, chaperones

b)

chaperones, chaperonins

c)

PDI enzymes, the ER

13.

PDI requires an __________ environment.

a)

oxidizing

b)

reducing

c)

positive

d)

negative

14.

Alzheimer's disease is associated with the improper folding of what protein?

a)

collagen I

b)

Huntingtin

c)

Amyloid Precursor Protein

d)

prions