WorksheetsEXPERIMENT 6: BASIC TECHNIQUE IN ISOLATING DNA
Total questions: 18
Worksheet time: 13mins
What is the purpose of using salt-detergent solution?
(the answer can be more than ONE)
Break up proteins
Emulsify fat
Create a polar environment to dissolve DNA
To denature protein found in the DNA
Ice chilled alcohol is used to
Breakdown DNA
Precipitate DNA
Breakdown cell wall of the fruits mixture
Separate components of the cell
The purpose of using water bath in this experiment is to
Extract DNA from the fruit mixtures
Denature protein that wound up the DNA
Emulsify fat
Prevent DNA from dissolving in aqueous solution
Why do we need to mash the fruits?
To breakdown cell wall of the fruits using mechanical force
To extract DNA from the fruits
To breakdown the fruits into smaller pieces
To breakdown plasma membrane of the fruits using mechanical force
Why extraction of DNA from plant cells nucleus is differ from animal cells nucleus?
(the answer can be more than ONE)
DNA from plant cells are easier to extract due to presence of cell wall
DNA from animal cells are easier to extract due to absence of cell wall
DNA from animal cells are more difficult to extract due to absence of cell wall
DNA from plant cells are more difficult to extract due to presence of cell wall
Enzymes that can be used to break the cell wall is
Catalase
Amylase
Cellulase
Hydrolase
The purpose of filtering the fruits mixture using muslin cloth is
Separate components of the fruits mixture
Separate components of the salt-detergent mixture
Separate components of the DNA
Separate components of the alcohol
Why do we need to drizzle the ice-chilled alcohol at flat angle?
To ensure the ice-chilled alcohol mix with the fruit extract
To ensure the ice-chilled alcohol mix with the salt detergent mixture
To ensure the ice-chilled alcohol does not mix wtih the salt detergent mixture
To ensure the ice-chilled alcohol does not mix with the fruit extract
What is the apparatus are used to peel the fruit?
(the answer can be more than ONE)
White tiles
Knife
Mortar
Spatula
Which is the precaution that must follow during mash the fruit?
Grind the fruit but not pouncing
Avoid the formation of bubbles
Must be in flat angle
Slowly pour the fruit
What is the temperature of water bath required to incubate the fruit mixture?
40OC
50OC
60OC
70OC
What is the apparatus used to sieve the fruits mixture to prevent formation of bubbles?
Muslin cloth
Mortar cloth
Filter paper
Glass rod
Where is the location of extracted DNA in boiling tube?
Between layers of alcohol and sieved liquid
Between layers of alcohol and salt detergent solution
Between layers of fruit mixture and sieved liquid
Between layers of fruit mixture and sieved alcohol
Why it is important for scientist to remove DNA from organism?
(the answer can be more than ONE)
To study about DNA sequence, structure and genome
To trace family lineage
To identify criminal suspects
To clone target genes in next child
Which are organelles in plant cells contain DNA?
Nucleus
Mitochondrion
Chloroplast
Ribosome
What is the major function of DNA?
Carry genetic information for growth
Carry codes for protein synthesis
Carry specific amino acid to form protein
Carry genes for cell division
What are the chromosome made of?
1 polynucleotide strand combine with histone
1 polynucleotide strand combine with histidine
2 polynucleotide strand combine with histone
2 polynucleotide strand combine with histidine
Why alcohol used must be in low temperature?
To increase yield of DNA extraction
To make sure DNA is not damage
To maintain the structure of DNA
To uncoil double helix DNA
