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Microscopy Quiz

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

Which part of the compound microscope helps in gathering and focusing light rays on the specimen to be viewed

a)

Eyepiece lens

b)

Objective lens

c)

Condenser lens

d)

Magnifying lens

2.

What is the minimum distance for the eye to focus any object?

a)

11 cm

b)

25 cm

c)

32 cm

d)

42 cm

3.

Resolving power of a microscope is a function of ____________

a)

Wavelength of light used

b)

Numerical aperture of lens system

c)

Refractive index

d)

Wavelength of light used and numerical aperture of lens system

4.

The greatest resolution in light microscopy can be obtained with ___________

a)

Longest wavelength of visible light used

b)

An objective with minimum numerical aperture

c)

Shortest wavelength of visible light used

d)

Shortest wavelength of visible light used and an objective with the maximum numerical aperture

5.

Oil immersion objective lens has an NA value of____________

a)

0.65

b)

0.85

c)

1.33

d)

1.00

6.

In fluorescence microscopy, which of the following performs the function of removing all light except the blue light?

a)

Exciter filter

b)

Barrier filter

c)

Dichroic mirror

d)

Mercury arc lamp

7.

Total Magnification is obtained by __________

a)

Magnifying power of the objective lens

b)

Magnifying power of eyepiece

c)

Magnifying power of condenser lens

d)

Magnifying power of both the objective lens and eyepiece

8.

Which part of the light microscope controls the intensity of light entering the viewing area?

a)

Coarse adjustment screw

b)

Fine adjustment screw

c)

Diaphragm

d)

Condenser lens

9.

If you were given a specimen of an active, motile microorganism, which of the following types of microscopy would be the most effective in visualizing the live microbe?

a)

bright-field microscopy

b)

dark-field microscopy

c)

fluorescence microscopy

d)

phase-contrast microscopy

e)

scanning electron microscopy

10.

The instrument that produces a bright image of the specimen against a dark background is called a__________ microscope

a)

phase-contrast

b)

transmission electron

c)

bright-field

d)

dark-field

11.

What do phase-contrast and dark-field microscopes have in common?

a)

They increase contrast between the specimens and their surroundings without staining.

b)

They make specimens appear dark on a bright background

c)

They make specimens fluoresce

d)

They make specimens visible that refract light away from the objective

12.

All of the following microorganisms can be observed with the bright-field microscope EXCEPT

a)

viruses

b)

fungi

c)

algae

d)

protozoa

e)

bacteria

13.

Which microscope lens does not magnify the imaged formed?

a)

magnetic lens

b)

ocular lens

c)

objective lens

d)

condenser lens

e)

none of the above

14.

If the minimum image size the human eye can detect is 0.1 mm, what is the minimum magnification needed to make an object measuring 1 um visible?

a)

10x

b)

100x

c)

1,000x

d)

10,000x

e)

100,000x

15.

What is the total magnification of a 10x ocular lens and a 45x objective lens?

a)

15x

b)

45x

c)

150x

d)

450x