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WorksheetsRECOMBINANT DNA TECHNOLOGY 2.0
Total questions: 21
Worksheet time: 21mins
- What is the function of the restriction enzyme for the bacteria themselves?
to cut their own DNA
to cut the invaded DNA
to cut open the plasmid
Choose the restriction enzyme that can do this
SmaI
PovII
BamHI
HaeIII
The function of restriction enzyme is to recognize and cut the specific sequence called (a)
the same restriction enzyme must be used to produce (a) ends.
the capacity of the DNA fragment is 10 kb. Choose TWO vectors that can be used
sCOS-1
pUC18
λ2001
pYAC
a DNA from hummingbird cell is cut at 10 recognition sites. How many fragments produced?
10
20
11
21
a DNA from humming bird is cut at 10 recognition site. How many fragment contains gene of interest?
10
11
1
0
Which of the following gene in the following plasmid having restriction site within to cut open the plasmid?
AmpR
ori
lacZ
choose the he sequence of sticky/ staggered end based on the diagram.
GCTTAA
CTTAA
TTAA
Choose the enzyme that can be used to seal the seal between plasmid and the inserted DNA fragment is
Taq polymerase
DNA ligase
EcoRI
SmaI
the uptake of the recombinant plasmid into the host cell is called
insertion
transformation
amplification
isolation
The unpaired nucleotides produced by the action of restriction enzymes are referred to as
sticky end.
base sequence.
blunt end
restriction fragments.
Which of the following palindromic base sequence identify by EcoRI
5' GATTTC 3'
5' GATTCC 3'
5' GAATCC 3'
5' GAATTC 3'
What is the name of an enzyme use in Polymerase Chain Reaction (PCR)?
reverse transcriptase
Taq polymerase
primase
During insertion step,
The recombinant plasmid is inserted into a host cell
The gene of interest is inserted into a plasmid
The plasmid is inserted into the gene of interest
