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WorksheetsApplication of Recombinant DNA Technology : Insulin
Total questions: 10
Worksheet time: 5mins
Name
Class
Date
1.
What is a cDNA?
a)
collection of total genomic DNA
b)
non-coding RNA molecule (containing about 22 nucleotides) found in plants,
c)
complementary DNA molecules synthesized from mRNA
d)
small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules
2.
Which of the following is/are true about reverse transcriptase
a)
works backwards, from DNA to RNA
b)
works forward from RNA to DNA
c)
works backwards from RNA to DNA
d)
works forward from DNA to RNA
3.
the function of reverse transcriptase is enzyme used to generate complementary DNA from an RNA template
a)
false
b)
true
4.
Human insulin produced by genetic engineering is better because (a) it acts faster (b) it lasts longer in the body (c) it does not cause allergic reactions
a)
a,b, and c
b)
a and c
c)
b and c
d)
a only
5.
The structure from which insulin mRNA can be obtained is ………………………………
a)
pancreas
b)
islets of Langerhans
c)
blood plasma
d)
liver
6.
in producing insulin by genetic engineering, what is the first step to take?
a)
isolate insulin from pancrease
b)
isolate insulin mRNA from pancreas
c)
isolate insulin DNA from pancreas
d)
isolate insulin from animal tissue
7.
mRNA is converted to cDNA by ………………
a)
Ligase enzyme
b)
Reverse Transcriptase enzyme
c)
Restriction Endonuclease enzyme
d)
RNA polymerase enzyme
8.
double stranded DNA is produced by ……………………….
a)
Ligase enzyme
b)
Reverse Transcriptase enzyme
c)
Restriction Endonuclease enzyme
d)
DNA polymerase enzyme
9.
Why is insulin produced by genetic engineering more rapid in its action?
a)
it is similar to animal insulin
b)
It has a shape that is exactly to that of natural human insulin
c)
It has higher optimum temperature of activity
d)
it is transported more rapidly by the blood to target sites
10.
What is the name of insulin produced by genetic engineering?
a)
Humulin G
b)
Humulin I
c)
Humulin H
d)
Humulin S
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