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Worksheets7.1 Methods in Gene Cloning
Total questions: 20
Worksheet time: 11mins
First stage involved in gene cloning.
Amplification of the target gene
Insertion of the target gene
Polymerase Chain Reaction
Isolation of the target gene
The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.
Cloning
insertion
Transformation
Isolation
Types of plasmids produced after insertion of the target gene into a vector.
Recombinant plasmids
Non-recombinant plasmids
Bacterial plasmids
Isolated plasmids
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
using recombinant DNA to produce human hormones from bacteria
testing parents for genetic disorders before they have children
engineering fruits and vegetables that resist insects and other pests
C. developing ways to identify criminals through DNA fingerprinting
How do scientists cut DNA into smaller strands?
restriction enzymes
scissors
ligase
agarose
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
Which enzyme "glues" DNA fragments together during insertion?
restriction enzymes
DNA ligase
DNA polymerase
DNA helicase
Which of the following could be used as a vector for a human gene?
Bacterial Plasmid
Bacteria
A mitochondria
mRNA
The site where restriction enzymes cut are a called:
cut sites
restriction sites
recognition sites
cleave sites
Sticky ends are produced when the restriction enzyme cuts in a:
staggered manner
straight through the DNA
Cell that receive recombinant DNA for cloning purpose
Host cell
plasmid
DNA target
Gene of interest
Which of the following is NOT a tool used in recombinant DNA technology?
Cloning vector
RNA polymerase
Target DNA
DNA ligase
Which of the following palindromic base sequence identify by EcoRI
5' GATTTC 3'
5' GATTCC 3'
5' GAATCC 3'
5' GAATTC 3'
What is recombinant DNA
DNA from 2 different organisms combined
DNA from 2 different ribosomes
DNA that is separated out from a vector
Recombinant DNA technology involves a screening process. What is the purpose of screening process?
Isolate recombinant DNA from bacteria
Identify cell with target DNA to be cut by restriction enzyme
Identify bacteria with plasmid to be used as vector
Identify bacteria with recombinant plasmid
Where are restriction enzymes naturally found?
in humans
in bacteria
in algae
they are not natural - all restriction enzymes are made in the lab
Plasmids often have genes that give the bacterial a survival advantage
True
False
What enzyme binds to the promotor of the plasmid to transcribe the gene inserted into the plasmid?
DNA polymerase
helicase
RNA polymerase
ligase
Which one of these components of a plasmid allow it to multiply independently?
Multiple cloning sites (MCS)
Origin of replication
Selectable marker
