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chem 2

Total questions: 50

Worksheet time: 25mins

Name
Class
Date
1.

Which of the following biochemical processes is promoted by insulin?

a)

Glycogenolysis

b)

Gluconeogenesis

c)

Lipolysis

d)

Uptake of glucose by cells

2.

Which of the following hormones promotes hyperglycemia?

a)

Calcitonin

b)

Growth hormone

c)

Aldosterone

d)

Renin

3.

Which of the following is characteristic of type 1 DM?

a)

Requires an OGTT for diagnosis

b)

Is the most common form of DM

c)

Usually occurs after age 40

d)

Requires insulin replacement to prevent ketosis

4.

Select the most appropriate adult reference range for fasting blood glucose

a)

40-105 mg/dL

b)

60-140 mg/dL

c)

65-99 mg/dL

d)

75-150 mg/dL

5.

When preparing a patient for an OGTT, which of the following conditions will lead to erroneous results?

a)

The patient remains ambulatory for 3 days prior to the test

b)

Carbohydrate intake is restricted to below 150 g/day for 3 days prior to test

c)

No food, coffee, tea, or smoking is allowed 8 hours before and during the test

d)

Administration of 75 g of glucose is given to an adult patient following a 10-12 hour fast

6.

Which of the following 2-hour glucose challenge results would be classified as impaired glucose tolerance?

a)

130 mg/dL

b)

135 mg/dL

c)

150 mg/dL

d)

204 mg/dL

7.

Which stationary phase is used for the measurement of HbA1c by HPLC?

a)

Octadecylsilane (C18)

b)

Cation exchanger

c)

Anion exchanger

d)

Polystyrene divinylbenzene

8.

What is the ADA recommended cutoff value for adequate control of blood glucose in diabetics as measured by glycated hemoglobin?

a)

5%

b)

6.5%

c)

9.5%

d)

11%

9.

What is the recommended cutoff for the early detection of chronic kidney disease in diabetics using the test for microalbuminuria?

a)

>30 mg/g creatinine

b)

>80 mg/g creatinine

c)

>200 mg/g creatinine

d)

>80 mg/L

10.

Which of the following is the reference method for measuring serum glucose?

a)

Somogyi-Nelson

b)

Hexokinase

c)

Glucose oxidase

d)

Glucose dehydrogenase

11.

Select the enzyme that is most specific for β-D-glucose

a)

Hexokinase

b)

G-6-PD

c)

Phosphohexisomerase

d)

Glucose oxidase

12.

Select the coupling enzyme used in the hexokinase method for glucose

a)

G6PD

b)

Peroxidase

c)

Glucose dehydrogenase

d)

Glucose-6-phosphatase

13.

Identify the enzyme deficiency responsible for type I glycogen storage disease (von Gierke's disease)

a)

Glucose-6-phosphatase

b)

Glycogen phosphorylase

c)

Glycogen synthetase

d)

β-Glucosidase

14.

Which of the following is classified as mucopolysaccharide storage disease?

a)

Pompe's disease

b)

von Gierke disease

c)

Hers' disease

d)

Hurler's syndrome

15.

PTH - Increased

Ca2+ - Increased

Phosphate - Decreased

a)

Primary hyperthyroidism

b)

Hypoparathyroidism

c)

NOTA

d)

Both

16.

TSH - Increased

Free T4 - Decreased

Free T3 - Decreased

a)

Primary hypothyroidism

b)

Secondary hypothyroidism

c)

Hyperthyroidism

d)

T3 thyrotoxicosis

17.

TSH - Decreased

Free T4 - Normal

Free T3 - Increased

a)

Primary hypothyroidism

b)

Secondary hypothyroidism

c)

Hyperthyroidism

d)

T3 thyrotoxicosis

18.

Major estrogen produced by ovaries

a)

Estradiol (E2)

b)

Estrone (E1)

c)

Estriol (E3)

19.

Cyclosporine and tacrolimus are examples of:

a)

Immunosuppressants

b)

Psychoactives

c)

Antineoplatics

d)

Antiepileptics

20.

Regulated by lungs

a)

Carbonic acid

b)

Bicarbonate

c)

AOTA

d)

NOTA

21.

Regulated by kidneys

a)

Bicarbonate

b)

Carbonic acid

c)

Either

d)

Neither

22.

Normal bicarbonate and carbonic acid ratio

a)

20:1

b)

10:1

c)

2:1

d)

5:1

23.

Match the correct parameter and device used


A. PCO2

B. PO2


1. Severinghaus electrode

2. Clark electrode

a)

A and 1; B and 2

b)

A and 2; B and 1

24.

Lactescence occurs when serum triglyceride level exceeds what concentration?

a)

25 mg/L

b)

25 mg/dL

c)

400 mg/L

d)

400 mg/dL

25.

Which of the following instruments is used in the clinical laboratory to detect beta and gamma emissions?

a)

Fluorometer

b)

Nephelometer

c)

Scintillation counter

d)

Spectrophotometer

26.

In potentiometry, the following are types of reference electrodes, EXCEPT:

a)

Glass electrode

b)

Standard hydrogen electrode

c)

Saturated calomel electrode

d)

Silver-silver chloride electrode

27.

Which of the following substances are introduced in a continuous-flow analyzer to minimize diffusion of reagents and mixing between samples?

a)

Membranes

b)

Resins

c)

Air bubbles

d)

Gel polymers

28.

The protein fraction that migrates the fastest toward the anode:

a)

Albumin

b)

Beta-globulin

c)

Alpha1-globulin

d)

Gamma-globulin

29.

Protein also known as orosomucoid:

a)

Alpha1-Acid glycoprotein

b)

Alpha2-Macroglobulin

c)

β-Lipoprotein

d)

C-reactive protein

30.

Which of the following substances is markedly increased in nephrotic syndrome?

a)

Ceruloplasmin

b)

Alpha-2-macroglobulin

c)

Alpha-1-antitrypsin

d)

Albumin

31.

The neocuproine method for glucoses is based on:

a)

Glucose oxidase reaction

b)

Copper reduction

c)

Condensation reaction

d)

Hexokinase reaction

32.

Select the enzyme most specific for beta D-glucose.

a)

Hexokinase

b)

Glucose-6-phosphate dehydrogenase

c)

Phosphohexisomerase

d)

Glucose oxidase

33.

All of the following are characteristics of Type II diabetes mellitus except:

a)

Insulin levels may or may not be abnormal

b)

It is more common than Type I diabetes

c)

It requires insulin therapy to control hyperglycemia

d)

It is associated with obesity and more common in persons greater than 40 years old

34.

Select the order of mobility of lipoproteins electrophoresed on cellulose acetate or agarose at pH 8.6.

a)

Chylomicrons → pre-beta → beta → alpha +

b)

Beta → pre-beta → alpha→ chylomicrons +

c)

Chylomicrons →beta → pre-beta → alpha +

d)

Alpha → beta → pre-beta → chylomicrons +

35.

Select the lipoprotein fraction that carries most of the endogenous triglycerides.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

36.

The lipoprotein that transports the exogenous triglycerides:

a)

HDL

b)

LDL

c)

VLDL

d)

Chylomicrons

37.

Apolipoprotein A is the primary protein component of:

a)

HDL

b)

LDL

c)

VLDL

d)

NOTA

38.

An abnormal lipoprotein found in patients with obstructive biliary disease:

a)

B-VLDL

b)

LpX

c)

Lp(a)

d)

LDL

39.

An abnormal LDL lipoprotein, also known as the “sinking pre-B-lipoprotein” associated with a higher risk for atherosclerosis:

a)

B-VLDL

b)

LpX

c)

Lp(a)

d)

LDL

40.

A type IIA hyperlipoproteinemia phenotype is associated with an increased level of:

a)

Chylomicrons

b)

HDL

c)

VLDL

d)

LDL

41.

The bilirubin fraction that is covalently attached to the albumin is:

a)

Direct

b)

Indirect

c)

Delta

d)

Bound

42.

In the diacetyl monoxime method, diacetyl reacts directly with:

a)

Urea

b)

Uric acid

c)

Ammonia

d)

Ammonium ion

43.

It absorbs light at 293 nm in the uricase method:

a)

Uric acid

b)

Uricase

c)

Allantoin

d)

NOTA

44.

Which of the following methods applies to the principle below?


Creatine + ATP → (CK) Creatine phosphate + ADP

a)

Szasz

b)

Wroblewski

c)

Tanzer-Gilvarg

d)

Oliver-Rosalki

45.

Which isoenzyme of creatine kinase (CK) has the fastest electrophoretic mobility at pH 8.6?

a)

MM

b)

MB

c)

BB

d)

Macro-CK

46.

Which of the isoenzymes of ALP is most anodal?

a)

Placental ALP

b)

Bone ALP

c)

Intestinal ALP

d)

Liver ALP

47.

Which enzyme is a useful indicator of possible insecticide poisoning?

a)

5'nucleotidase

b)

ACP

c)

Cholinesterase

d)

LDH

48.

Which of the isoenzymes of ALP is most heat stable?

a)

Placental ALP

b)

Bone ALP

c)

Liver ALP

d)

Intestinal ALP

49.

There are several alkaline phosphatase isoenzymes that are characteristic of cancer. Electrophoretic mobility of these fractions is similar to what ALP isoenzyme:

a)

Placental ALP

b)

Bone ALP

c)

Liver ALP

d)

Intestinal ALP

50.

The Szasz assay determines the activity of:

a)

CK

b)

ALP

c)

GGT

d)

LDH

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