WorksheetsPCR
Total questions: 10
Worksheet time: 6mins
Name
Class
Date
1.
What is the purpose of PCR?
a)
To produce millions of copies of DNA.
b)
To produce millions of copies of a specific region of DNA
c)
To add nucleotides to a DNA sequence
d)
To watch polymerase work.
2.
At what temperature does the Denature step of PCR occur?
a)
94-96° C
b)
50-65° C
c)
72° C
d)
Any temperature
3.
Why does PCR require a specific type of polymerase?
a)
Taq polymerase can withstand the range of temperatures in PCR without denaturing.
b)
Taq polymerase is the most inexpensive type of polymerase.
c)
Taq polymerase is the most common type of polymerase.
d)
A specific type of polymerase is unnecessary.
4.
The first step in PCR is
a)
Primer annealing
b)
Denaturation
c)
Primer extension
d)
None of these
5.
What happens during the Anneal step of PCR?
a)
Primers are created.
b)
The primers attach to the target DNA region.
c)
Primers copy the new DNA strand.
d)
Primers sequence DNA
6.
Does PCR require a primer?
a)
Yes
b)
No
7.
What is the denaturation step?
a)
mutating RNA polymerase
b)
Cooling DNA to allow primers to form
c)
separating DNA helix in a cold environment
d)
separating DNA helix with using heat
8.
What is the Extension step?
a)
DNA polymerase adds nucleotides to the 5' end
b)
RNA polymerase adds nucleotides to the 5' end
c)
DNA polymerase adds nucleotides to the 3' end
d)
RNA polymerase adds nucleotides to the 3' end
9.
What process directly follows PCR?
a)
gel electrophoresis
b)
DNA sequencing
c)
cloning
d)
transformation
10.
What is the purpose of the Extension step of PCR?
a)
To create the primers.
b)
To extend the time it takes to produce DNA.
c)
To allow polymerase to create the complementary strands of DNA.
d)
To allow substrates to copy DNA sequences.
100 %
