NEW
Font size
WorksheetsPBS 2020 Quiz 3 Unit 1 DNA Analysis
Total questions: 28
Worksheet time: 14mins
DNA is a long chain made up of smaller subunits called
nucleotid
nucleotides
Double helix
Restriction enzymes
What is the purpose of PCR?
To amplify DNA (make lots of copies of DNA)
Make Double helixs
The genetic makeup of an individual
To break apart the fat in the cell membrane
When running a gel, DNA will move to the ____ end because the DNA has a ____ charge
Double / helix
mashing / individual
positive / negative
cytosine,/ thymine
A nucleotide is made up of a sugar, a phosphate, and a _____
Restriction enzyme
positive molecule
nitrogenous base
polymerase chain
Where is DNA found?
with base pairs
in a Double helix
the nucleotides
the nucleus
DNA is and does what?
strong bonds that hold nucleotides together
multiple genes put together to form an organism
ribonucleic acid. Sequence of nucleotides arranged in a specific order, contains instructions for a living organism
Deoxyribonucleic acid. Sequence of nucleotides arranged in a specific order, contains instructions for a living organism
The pentagon represents...
Five carbon sugar
the nucleus
Adenine and Guanine
Phosphate
A Gene is...
Deoxyribonucleic acid
multiple genes put together to form an organism
the sequence of nucleotides
sequence of nucleotides that code for a specific protein
A Genome is....
Deoxyribonucleic acid
Purines: Adenine and guanine
Pyrimidines: Cytosine, Thymine, and Uracil
a nucleotide
multiple genes put together to form an organism. These are in the same order for each species of organism
What are the steps of gel electrophoresis?
1. Make the gel
2. Set up the gel apparatus
3. Load the DNA sample into the gel
4. Hook up the electrical current and run the gel
5. Stain the gel and analyze the results
Purines: Adenine and guanine
Pyrimidines: Cytosine, Thymine, and Uracil
A solution of DNA molecules is placed in a gel.
1. move extracted DNA into PCR tube
2. add primer 1 to PCR tube
3. add primer 2
4. add nucleotide to the PCR tube
5. add DNA polymers to the PCR tube
6. Place DNA PCR tube into DNA thermal cycler
How do you read the gel in gel electrophoresis?
By Placing DNA PCR tube into DNA thermal cycler
By using Restriction enzymes
Each set of DNA is in its own "lane". Once you have completed the steps of DNA use the DNA marker (ruler) to measure the DNA. Compare the DNA by looking at how similar or different each of the "lanes" is
Stain the gel and analyze the light colored results
What is the structure of DNA?
the nucleus
nucleotides
Double helix
Adenine and Guanine
Restriction enzymes HaeIII, cuts DNA between which base pair sequence?
AA/TT
GG|CC
GC/GC
TA/AT
To create recombinant DNA, foreign DNA is inserted into _____ DNA
Virus
Human
Bacteria
Fish
What are the Steps of PCR ?
Only one, To amplify DNA (make lots of copies of DNA)
1. Make the gel
2. Set up the gel apparatus
3. Load the DNA sample into the gel
4. Hook up the electrical current and run the gel
5. Stain the gel and analyze the results
1. Denaturation
2. Annealing
3. Extension
No steps, because strong bonds that hold nucleotides together
Gel electrophoresis purpose is ....
To amplify DNA (make lots of copies of DNA)
to make Purines: Adenine and guanine
A solution of DNA molecules is placed in a gel. Because each DNA molecule is negatively charged, it can be pulled through the gel by an electric field. Small DNA molecules move more quickly through the gel than larger DNA molecules
technique for sorting molecules or fragments of molecules by length. It is used to place someone at a crime scene, figure ot relatives, etc
Why are we different?
Purines: Adenine and guanine attack Pyrimidines: Cytosine, Thymine, and Uracil
We have strong bonds that hold nucleotides together
the sequence of nucleotides within each gene have changes
we break apart the fat in the cell membrane differently
How does gel electrophoresis work?
by Staining the gel
to place someone at a crime scene
The genetic makeup of an individual
A solution of DNA molecules is placed in a gel. Because each DNA molecule is negatively charged, it can be pulled through the gel by an electric field. Small DNA molecules move more quickly through the gel than larger DNA molecules
How many hydrogen bonds are between A and T?
2
3
1
4
Outline the process of PCR
1. move extracted DNA into PCR tube
2. add primer 1 to PCR tube
3. add primer 2
4. add nucleotide to the PCR tube
5. add DNA polymers to the PCR tube
6. Place DNA PCR tube into DNA thermal cycler
No process, just a technique for sorting molecules or fragments of molecules by length
A solution of DNA molecules is placed in a gel. Because each DNA molecule is negatively charged, it can be pulled through the gel by an electric field. Small DNA molecules move more quickly through the gel than larger DNA molecules
To amplify DNA (make lots of copies of DNA)
If Guanine makes up 20% of the DNA, what percentage would be Cytosine?
20%
30%
60%
Not enough information given
What are the three parts of a nucleotides?
James, Watson and Crick
Bacteria, virus, and fungus
Nitrogenous base, 5-carbon sugars, phosphate group
Nitrogenous base, 6-carbon sugars, phosphate group
Adenine and Thymine are considered to be...
Bacteria
Discovered the structure of DNA, but her discovery was credited to Watson and Crick
polymerase chain
base pairs
PCR stands for
polymerase chain reaction
amplify DNA
Deoxyribonucleic acid
Peoxyribonucleic Carbon reaction
Which two scientist are credited with discovered DNA?
Whatson and Crock
Nitrogenous base, and 5-carbon sugars
Watson and Crick
Guanine and Cytosine
A monomer is ...
base pairs
a lone Bacteria
nitrogenous base
nucleotide
What is the smallest component of DNA?
an atom
Nitrogenous base
5-carbon sugars
phosphate group
Which nitrogenous bases are pyrimidines?
nitrogenous base
Adenine and guanine
cytosine, thymine, uracil
5-carbon sugars
