NEW
Font size
WorksheetsGenetic Engineering
Total questions: 25
Worksheet time: 13mins
DNA strands can be clipped crosswise at selected positions by using enzymes called
palindromes
DNA polymerases
restriction endonucleases
ligases
Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?
genetic engineering
PCR
antisense technology
microarray analysis
The polymerase chain reaction (PCR) has been used in all of the following fields, except
gene mapping
forensics
medicine
none of the choices; it is used for all these fields
Which PCR step causes the denaturation of double-stranded DNA?
Repeat the cycle of heating and cooling
Add primers
Heat target DNA to 94 degrees Celsius
Cool DNA to between 50 degrees and 65 degrees Celsius
The use of an organism's biochemical processes to create a product is
Bioremediation
gene probes
biotechnology
recombinant DNA
Transgenic animals
will pass genes on to their offspring
commonly include mice
can be engineered to become factories for manufacturing proteins
All of these choices are correct
Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects.
Pseudomonas fluorescens
Thermus aquaticus
Escherichia coli
Saccharmonyces cerevisiae
All of the following are correct about DNA fingerprinting, except
it cannot be used on specimens older than five years
it can be used to analyze evidence when only minute amounts of DNA are found
it is routine to use it as trial evidence in the U.S.
it displays the unique pattern of an individual's DNA fragments
Which of the following drugs is/are produced by genetic engineering and approved for human use?
tissue plasminogen activator
Factor VIII
Human growth hormone
All of these choices are correct
Amplification of DNA is accomplished by
Southern blot
polymerase chain reaction
gene probes
All of these choices are correct
Restriction endonucleases are obtained from various species of bacteria.
True
False
Collections of isolated genes maintained in a cloning host is known as
transgenic organisms
Genomic libraries
cloning vectors
CRISPR
The deliberate removal of genetic material from one organism and combining it with a genetic material of another organism is a specific technique called
genetic engineering
gel electrophoresis
recombinant DNA technology
gene probes
Which of the following regions of DNA would be of most value for forensic analysis?
DNA sequences that are typically identical for all humans
Most DNA sequences are equally valuable for forensic analysis
DNA sequences that differ among humans, with a maximum of two different alleles
SNa sequences that are quite variable with multiple different alleles
Sequencing a gene automatically identifies which gene it is.
True
False
All of the following are desirable features in a microbial cloning host, except
the host has a slow growth rate
the host will secrete a high yield of proteins from expressed foreign genes
the host can be grown in large quantities
the host is capable of accepting plasmid vectors
The Western Blot technique detects
recombinant DNA
specific genetic marker sequence on genes
DNA
proteins
Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?
antisense therapy
DNA fingerprinting
microarray analysis
gene mapping
Reverse transcriptase is used to make cDNA from an RNA template.
True
False
An example of gene therapy is the insertion of the gene for human growth hormone in E. coli cells.
True
False
When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called
gene therapy
DNA fingerprinting
cloning
antisense therapeutic
Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using
palindromes
reverse transcriptases
ligases
restriction endonucleases
A technique that separates a readable pattern of DNA fragments is
gene probes
gel electrophoresis
biotechnology
recombinant DNA
When DNA is heated, the two strands will separate.
True
False
Which of the following would be of LEAST value in DNA profiling?
STR short tandem repeat) analysis
sequencing blood group alleles
analysis of SNPs (single nucleotide polymorphisms)
mitotyping
