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WorksheetsColumn Chromatography 1
Total questions: 9
Worksheet time: 5mins
It is a technique or method used to separate mixtures of dissolved chemicals/substances.
chromatography
solution
chromatogram
evaporation
In size exclusion chromatography, the higher the degree of cross-linking of the gel polymer, ………..
the lower the mechanical strength and the larger the molecular sieving range.
the higher the mechanical strength and the larger the molecular sieving range.
the lower the mechanical strength and the smaller the molecular sieving range.
the higher the mechanical strength and the smaller the molecular sieving range.
In gel permeation chromatography, a type of size exclusion C, which of the following is correct?
Compressible gels are commonly used.
Spherical bead shape is preferred.
Mechanical strength increases with large particle size.
Gels with large bead size are preferred.
Q. Chromatography is generally used for the separation of mixtures based on the differences in their
shape
size
structure
all of the above
Ion-exchange chromatography is used for the separation of
polar molecules
nonpolar molecules
none of the above
all of the above
Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?
ion exchange chromatography
affinity chromatography
adsorption chromatography
igel permeation chromatography
In gel filtration (size exclusions) chromatography, separation of proteins are based on their
size and net charge
size and shape
size and specific affinity
shape and net charge
Which polymer is often used for matrix materials in Affinity chromatography
Agarose
cellulose
dextrose
all of the above
In anion exchange chromatography,
the column contains positively charged beads where negatively charged proteins bind
the column contains negatively charged beads where positively charged proteins bind
the column contains negatively & positively charged beads where proteins bind depending on their net charge
all of the above
