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Unit 3 MLT 140 Review

Total questions: 25

Worksheet time: 13mins

Name
Class
Date
1.
Asacchrolytic organisms
a)
ferment maltose only
b)
ferment glucose only
c)
ferment all carbohydrates
d)
ferment no carbohydrates
2.
You subcultured your Neisseria organisms yesterday and placed them in an anaerobic environment with an increase of CO2. Today, there was no growth on the media. What happened?
a)
You did not put enough organism on the plate.
b)
You should have incubated the plate in an aerobic environment. Neisseria species are aerobes.
c)
24 hours was too long of an incubation and the organisms died
d)
Neisseria should not be incubated in an increased amount of CO2.
3.
Haemophilus species have the following Gram stain pattern.
a)
GNDC
b)
GPC Cl
c)
GNCB
d)
GPR
4.
Your coworker asked you to read her stack of genital culture direct Gram stain smears. Which of the following should be be very careful to look for in the Gram stain?
a)
GPDC
b)
GNCB
c)
GNDC
d)
GPC Cl
5.
You are training a new employee in the identification of Neisseria meningitidis. Which of the following would be correct information to provide the new employee about identification of the organism?
a)
All Neisseria should be incubated without Oxygen.
b)
Neisseria meningitidis ferments all carbohydrates.
c)
Neisseria meningitidis ferments glucose and maltose.
d)
The Gram stain of Neisseria meningitidis is GNCB intra and extracellular.
6.
You are reading cultures from the respiratory bench this morning. Which of the following cultures would you expect to NOT contain normal flora?
a)
Bronchial washing from the lower respiratory tract
b)
Throat culture
c)
Nasopharyngeal culture
d)
Sputum that has bee coughed up by the patient
7.
Why is it important that Neisseria gonorrheae be collected on a JEMBEC plate if the culture cannot be immediately transported to the lab?
a)
The JEMBEC plate contains a battery and can start incubation immediately.
b)
The JEMBEC plate contains BAP and will create a CO2 enriched environment prior to transport to the lab.
c)
The JEMBEC plate contains MTM and can create a humid, CO2 enriched environment prior to transport to the lab.
d)
JEMBEC plates are not used for this organism.
8.
Modified Thayer Martin is Chocolate agar that contains
a)
Vancomycin, Colistin, Nystatin, and Trimethoprim
b)
Cephalosporin, Colistin, Nystatin, and Trimethoprim
c)
Vancomycin, Cephalosporin, Nystatin, and Trimethoprim
d)
Vancomycin, Colistin, Nystatin, and Cephalosporin
9.
Your new coworker screamed across the lab to tell you that she has a round, white, creamy, opaque colony on BAP. She needs help identifying it. What advice do you give her? Choose the best answer.
a)
"Sounds like a Staphylococcus species to me. Start with a Catalase test."
b)
"It could be Group A Strep. Make a CAMP test."
c)
"You may have Neisseria. What is the oxidase result?"
d)
"That should not grown on BAP. Are you sure you are looking at BAP and not CHOC?"
10.
To isolate the organism that is the causative agent of GUD/Chanchroid, which media should you include in the gential culture?
a)
MTM
b)
CHOC
c)
Nairobi plate
d)
MAC
11.
When reading a CSF culture, which of the following sets of organisms should you purposefully take time to look for in Gram stain?
a)
GPC cl and GPC ch
b)
GPC ch, GNCB, and GNDC
c)
GNCB only
d)
GNDC
12.
Haemophilus species with the prefix "para" are special because
a)
they can make their own NAD
b)
they can make their own V factor
c)
they can make their own Glucose
d)
the can make their own Hemin
13.
Which body site is considered to be sterile?
a)
Eye
b)
Throat
c)
Large intestine
d)
Genital area
14.
All Neisseria species are
a)
maltose fermenters
b)
asacchronlytic
c)
oxidase positive
d)
GNCB
15.

Read the carbohydrate fermentation results from this GNDC organism and choose the correct identification.

a)

Neisseria meningitidis

b)

Neisseria gonorrhoeae

c)

Haemophilus influenzae

d)

Moraxella catarrhalis

16.

Gram-positive bacteria include:

a)

staphylococci

b)

gonococci

c)

enterococci

d)

meningococci

e)

streptococci

17.

Streptococcus is:

a)

gram-negative diplococci

b)

gram-positive tetracocci

c)

gram-positive cocci in the form of a bunch of grapes

d)

gram-negative cocci

e)

gram-positive cocci located in a chain

18.

The morphology of meningococci is characterized by:

a)

In pairs are located

b)

Bean-shaped cocci

c)

Gram-negative

d)

Formed a capsule

e)

Movable

19.

Read the Gram stain

a)

GPC in clusters

b)

Gram positive cocci in chains

c)

GNR

d)

GNCB

20.

Read the Gram stain

a)

GPC in cl

b)

GPC in ch

c)

GNR

d)

GNCB

21.

Read the Gram stain

a)

GPC in cl

b)

GNCB

c)

GNDC intra and extracellular

d)

GNCB

22.

Read the sugar fermentation and choose the organism.

a)

Neisseria meningitidis

b)

Neisseria gonorrhoeae

c)

S. aureus

d)

S. pyogenes

23.

All Neisseria species are oxidase positive.

a)

True

b)

False

24.

All Neisseria species are catalase negative.

a)

True

b)

False

25.

Which media will aid in the isolation of N. gonorrhoeae

a)

CHOC

b)

BAP

c)

MAC

d)

MTM

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