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Microbiology for Patho

Total questions: 37

Worksheet time: 27mins

Name
Class
Date
1.

Counter stain used in Gram's Staining is

a)

Crystal Violet

b)

Ethanol

c)

Safranin

d)

Malachite green

2.

Mordant used in Gram's Staining is

a)

Crystal Violet

b)

Safranin

c)

Ethanol

d)

Iodine

3.

Which of the following statement is TRUE regarding Gram positive bacteria?

a)

Cell wall have high lipid content

b)

Lipopolysacharides layer is present

c)

crystal violet stain is retained

d)

Have 2 layers of cytoplasmic membrane.

4.
Which is true about bacteria cells ?
a)
they lack lipids
b)
they lack a nucleus
c)
they lack proteins 
d)
they lack DNA
5.
This scientist is best known for his Nobel Prize-winning discovery of the antibiotic penicillin in 1928.
a)
Alexander Fleming
b)
Albert Einstein
c)
Louis Pasteur
d)
Joseph Lister
6.

This scientist, known for his “Germ Theory,” showed that airborne microbes were the cause of disease. The Father of Microbiology.

a)

Alexander Fleming

b)

Francesco Redi

c)

Louis Pasteur

d)

Joseph Lister

7.
A prominent difference between prokaryotic and eukaryotc cells is the..
a)
larger size of the prokaryotes
b)
lack of pigmentation in eukaryotes
c)
presence of a nucleus in eukaryotes
d)
presence of a cell wall in prokaryotes
8.

This type of media inhibits the growth of organisms other than the one being grown. Can be complex or chemically defined media.

a)

selective media

b)

diferential media

c)

chemically defined media

d)

enrichment culture media

9.

This type of media contains specific ingredients that help us to visually distinguish which species do or do not carry out a specific biochemical process.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

10.

T/F: media can be both selective and differential.

a)

True

b)

False

11.

This media selects for gram-negative organisms and isolates lactose fermenters (pink) and non-lactose fermenters (clear).

a)

choclate agar

b)

Mannitol salt agar

c)

Eosin-methylene blue (EMB) agar

d)

MacConkey agar

12.

This is a general purpose medium that supports the growth of a wide variety of bacteria.

a)

blood agar

b)

nutrient agar

c)

chocolate agar

d)

MacConkey agar

13.

What type of hemolysis is presented on the Blood Agar

a)

Beta

b)

alpha

c)

gamma

d)

none

14.
Which media is differential but not selective?
a)
Blood Agar plate
b)
Columbia CNA
c)
Mac Conkey’s
d)
TSA
15.

The following media will allow growth of GN bacteria EXCEPT:

a)

Mac Conkey’s

b)

EMB

c)

Blood

d)

MSA

16.
When an organism growing on MSA ferments mannitol, it will appear:
a)
Pink
b)
Dark red
c)
Yellow
d)
Green
17.

Which of the following is the CORRECT order of the steps of a Gram stain?

a)

Primary stain → Decolorizing agent → Mordant → Counterstain

b)

Counterstain→ Mordant → Decolorizing agent→ Primary stain

c)

Primary stain→ Mordant→ Decolorizing agent→ Counterstain

d)

Primary stain→ Counterstain→ Decolorizing agent→ Mordant

18.

Gram positive bacteria will be stained in __________ color after Gram staining procedure?

a)

Red

b)

Pink

c)

Blue

d)

Purple

19.
Which part of a bacteria cell wall "holds" the stain? 
a)
plasma membrane
b)
fimbriae
c)
peptidoglycan
d)
pilus
20.
These bacteria are
a)
gram positive
b)
gram negative
c)
nonliving
d)
viral
21.

This is

a)

gram positive

b)

gram negative

c)

Mycobacterium

d)

Who knows? (don't choose)

22.

Which of the following questions largely stimulated the research of microbes during what is known as the Golden Age of Microbiology?

a)

How should living organisms be classified?

b)

What causes disease, and is spontaneous generation of microbes possible?

c)

How are microbes related?

d)

How can microorganisms be seen?

23.

Arisototle was an early natural philosopher who formulated what

a)

Germ theory of disease

b)

theory of natural selection

c)

theory of "magic-bullets"

d)

theory of spontaneous generation (abiogenesis)

24.

Identification of bacteria in the lab usually begins with the ___________ for placement in on of two categories of bacteria

a)

Petri stain

b)

Koch's stain

c)

Gram stain

d)

Pasteur fermentation test

25.

The work of Lister and Semmelweis contributed to controlling infectious disease by -

a)

identifying the sources of infectious agents

b)

determining the taxonomic relationships among microbes

c)

developing techniques for isolating pathogens

d)

developing methods for reducing health care associated infections (HAI) through aseptic technique

26.

Whose search for chemicals that would kill microbes without harming humans was the foundation for chemotherapy and the cure for syphilis?

a)

Lister

b)

Ehrlich

c)

Kock

d)

Pasteur

27.
Bacilli bacteria are what shape?
a)
spiral
b)
rod
c)
round
28.

Which phase in bacterial growth curve shows the reproduction rate equal to the death rate?

a)

Lag Phase

b)

Stationary Phase

c)

Log Phase

d)

Decline Phase

29.

What is the first stage of microbial growth?

a)

Lag

b)

Death

c)

Log

d)

Steady

30.

Read the Gram stain

a)

GPC in clusters

b)

Gram positive cocci in chains

c)

GNR

d)

GNCB

31.

This gram stain has what morphology and possible organism?

a)

GNR, S. aureus

b)

GPR, M. luteus

c)

GNR, E.coli

d)

GPR, Lactobacillus

32.

This gram stain has what morphology and what possible organism?

a)

GPR, clusters, S. aureus

b)

GPC, tetrads, M. luteus

c)

GPC, clusters. S. aureus

d)

GPC, tetrads, S. aureus

33.

What is the gram stain morphology and what is the possible organism?

a)

GPC, clusters, M. luteus

b)

GPR, clusters, S. aureus

c)

GPC, clusters, S. aureus

d)

GPR, Clusters, M. luteus

34.

The acid fast stain for Mycobacterium is used to stain...

a)

peptidoglycan layers

b)

bacteria with capsules

c)

lipopolysaccharide layers

d)

bacteria with waxy cell walls with mycolic acid

35.

Gram negative bacteria have

a)

lipopolysaccharide layer

b)

peptidoglycan layer

c)

mycolic acid layers

d)

lipopolysaccharide and peptidoglycan layers

36.

Examples of antibiotic mis-use would be all of the following except?

a)

taking the medication until you feel better and then for two more days to be effective

b)

using your mom's medication because she had the same infection

c)

taking the 14 day course of the 14 day prescription

d)

doctors prescribe antibiotics to treat your viral infection with great success

37.

Organisms that require an enhanced environment with decreased oxygen and increased CO2

a)

aerobic

b)

anaerobic

c)

microaerophilic

d)

facultative anaerobe.

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