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WorksheetsBiotechnology - UNIT-I
Total questions: 25
Worksheet time: 13mins
Use of biological organisms for the replacement of existing chemicals or production of new products with quality, high quantity in a short period is________.
Biotechnology
Biochemistry
Genetics
Biology
Give an example for restriction enzymes
EcoRI
Ligase
Reverse transcriptase
klenow enzyme
Sticky end cut restriction enzyme is ____
BamHI
EcoRI
pBR322
TiPlasmid
Important Scope of biotechnology is
Improving the financial status of the human being
Improving the quality of human life and at protecting him from dangerous diseases.
Improving the population of wild animals
Improving the population of wild flora
ICGEP (International Centre for Genetic Engineering and Biotechnology) located at
Bangalore
New Delhi
Chennai
Hydrabad
Molecular pharming pharming is the use of genetically modified plants (or animals) as a source of pharmaceutical products.
True
False
The DNA fragments have sticky ends due to
Endonuclease
Calcium ions
Unpaired bases
Free methylation
Restriction site for EcoRI is
True
False
Gene cloning vector is
An animal carries pathogen and spread to other animals
A DNA carries another DNA and inserted to host cell
It carries a particular disease from one living thing to another
None of the above
cDNA obtained from
T DNA
rDNA
Z DNA
mRNA
cDNA synthesized with the help of
Reverse transciptase
rRNA
Polymerase
SI nuclease
Which of the following is used for genetic engineering?
Type I Restriction Enzyme
Type II Restriction Enzyme
Type III Restriction Enzyme
All of the above
__________ is used for joining of two DNA fragments.
Ligase enzyme
Restriction enzyme
EcoRI
Bam HI
Taq Polemerase is isolated from _______
E.coli
Pseudomonas
Retrovirus
Thermus aquaticus
Plasmids are used as cloning vectors for which of the following reasons?
Presence of ORI gene
Presence of genetic markers
Capacity of insertional inactivation
All of the above
pBR 322 is
A bacterial vector
An Animal vector
A Plant vector
A viral Vector
Ti plasmid is _____
An Animal vector
A plant vector
A Bacterial vector
None of the above
TDNA present in
pBR 322
PUC 8
Ti plasmid
Col E1
T DNA has
coding region for synthesis of auxin
coding region for synthesis of cytokinin
coding region for synthesis of Opine
All of the above
Vir region of Ti plasmid is used for integration of TDNA with DNA of plant cell.
False
True
Ti plasmid is present in
Lacto basillus
Pseudomonas
E.coli
Agrobacterium tumefaciens
SV40 is
an animal vector
a plant vector
a bacterial vector
a plasmid vector
Ti Plasmid induced tumour to
Monkey
Man
Monocotyledon plant
Dicotyledon plant
Why need vector?
Gene cloning vector prevent the damage of target DNA by cellular enzyme of host cell.
Increase the Expression of target DNA
All of the above
Simian virus 40 was first isolated from
Monkey
Man
Plant
E.coli
