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WorksheetsPractical-1, Enzyme Purifcation
Total questions: 10
Worksheet time: 5mins
What is the source of enzyme?
Acid Phosphatase
Alkaline phosphatase
Moong bean sprouts
Liver tissue
What is the enzyme you are purifying?
Acid phosphatase
Alkaline phosphatase
Phosphohexose isomerase
Glycogen phosphorylase
What is the sequence of purification?
grinding-filtering--dialysis-centrifugation-precipitation
centrifugation-grinding-precipitation-dialysis-filtering
grinding-filtering-centrifugation-precipitation-dialysis
dialysis-grinding-centrifugation-precipitation-filtering
What salt is used salt fractionation in your experiment?
Aluminum sulphate
Ammonium Sulphate
Sodium chloride
Sodium sulphate
how long one should keep the sample for dialysis normally
1 hr
10 hr
24 hrs
48 hrs
This is the chief principle of Dialysis
Capillary action
Adhesion
Osmosis
Cohesion
What is the composition of the membrane that is used in Dialysis?
Chitin
Polyethylene
Polyvinyl chloride
Cellulose
Proteins are stored in
Sodium Azide
At ultra low temperature with cryoprotectant
In lyophilized form
All of the above
What is homogenization
Removing the cell debris
Centrifugating the samples
Making a homogenous mixture of sample and buffer
None of the above
What is the benefit of partial purification?
Less steps are required
Less time consuming
Cost effective
All of the above
