WorksheetsMICROBIOLOGY 1ST ASSESSMENT
Total questions: 46
Worksheet time: 30mins
Who disproved the Spontaneous generation theory of microbes?
Paul Ehrlich
Good Pasture
Louis Pasteur
Joseph Lister
What staining is this?
Acid fast staining
Gram staining
Albert's staining
Negative staining
Which microscope is used to view extremely slender microorganisms?
Fluorescent microscope
Phase contrast microscope
Electron microscope
Dark field microscope
Metachromatic granules appear ___________in Albert’s stain?
Red
Green
Blue
Bluish black
Who discovered Polymerase Chain reaction?
(a)
Write the Name and Type of this culture medium
(a)
Resolving power of a microscope depends on
The focal length and aperture of the eye lens
The focal length and aperture of the objective lens
The aperture of the objective and the eye lens
The wavelength of light illuminating the object
Principal centers of respiration in bacteria
(a)
Who discovered Lepra bacillus?
Robert Koch
Ivanovosky
Frankel
Hansen
Loeffler’s serum slope is used for growth of which bacteria?
(a)
Shadow casting technique is used in which microscope?
(a)
Chemically the bacterial capsule may be
Polypeptide
Polysaccharide
Either a or b
None of these
Stuart’s transport medium is used for
Streptococci
Meningococci
Gonococci
Vibrio
Write the name of this culture medium and it its use.
(a)
Disposal plastic syringes are sterilized by
Hot air oven
Radiation
Autoclave
Boiling
Identify this culture media
(a)
Generation time for Mycobacterium tuberculosis
20 hrs
2 days
2 min
20 min
Identify this culture media
(a)
Disinfection of enclosed areas [laboratories, ICU] can be done by using
UV radiation
Formaldehyde
Alcohol
Hypochlorite
What type of hemolysis seen in this blood agar plate?
(a)
The outer membrane of the Gram-negative cell wall is anchored to the underlying peptidoglycan by means of which of the following?
Braun’s Lipoprotein
Phospholipids
Proteins
Lipopolysaccharide
Which one of the following is true?
Agar has nutritive value
Agar melts at 45˚C and solidifies at 92˚C
Robertson’s cooked meat medium is used for growing aerobes
Biphasic media is used for blood culture
pH of this media
5.5
7.2
7.6
8.5
The location where the bacterial chromosomes concentrates is called
Nucleus
Nuclein
Nucleoid
Nucleose
The efficiency of moist-heat sterilization is tested with
Clostridium botulinum
Vegetative cells of Bacillus stearothermophilus
Spores of Bacillus stearothermophilus
Spores of aerobic bacteria
Write the phases of bacterial growth curve in order
(a)
Write the name of the inner tube present in this media and its use
(a)
Method of Sterilization for Lowenstein Jensen media
Autoclave
Boiling
Inspissation
Tyndallisation
Heating
Choose the instrument used to sterilize Glass articles
Choose the right order of flagellar arrangement for the given picture
Amphitrichous, Lophotrichous, Peritrichous, Monotrichous
Monotrichous, Amphitrichous, Peritrichous, Lophotrichous
Peritrichous, Amphitrichous, Lophotrichous, Monotrichous
Monotrichous, Lophotrichous, Amphitrichous, Peritrichous
Which microscope shows the given view
Electron microscope
Fluorescent microscope
Compound microscope
Phase contrast microscope
Write the name of this staining method and which part of bacterial cell can be demonstrated by using this?
(a)
Choose the appropriate morphology for the given bacteria
Gram negative bacilli
Gram positive cocci arranged in chains
Gram positive bacilli arranged in chains
Gram positive branching filamentous bacteria
Endospore are all of the following except ___________ as compared to vegetative cells.
more likely to survive treatment with disinfectants
more resistant to staining
more likely to die in nutritionally poor conditions
more resistant to temperature changes
In what phase of the growth curve does a culture have equal no's of dividing and dying cells
(a)
Peptidoglycan is made up of
N-acetylglucosamine
N-acetylmuramic acid
N-acetylglucosamine, N-acetylmuramic acid
N-acetylglucosamine, N-acetylmuramic acid, amino acids
Which of the following method can be used to determine the number of bacteria quantitatively?
Streak-plate
Spread-plate
Pour plate
Pour-plate and spread plate
The L Ring in Gram-Negative bacterium flagella is associated with
Peptidoglycan
Outer Membrane
Cytoplasmic Membrane
Cell Membrane
Which culture method is used in this picture
(a)
Write the name of this instrument
(a)
Identify this culture method
(a)
What are the blue color structures seen in this picture?
(a)
What is the temperature and time required for sterilizing sharp instruments in Hot air oven?
160 C for 1 hour
160 C for 2 hour
150 C for 1 hour
150 C for 2 hour
Write the expansion of HEPA filter
(a)
Endoscopes and bronchoscopes are sterilized by
Autoclave
Hot air oven
Glutaraldehyde
Formalin
Arthroscopes and Urethroscopes are sterilized by
(a)
