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WorksheetsOne Dimensional Protein Electrophoresis
Total questions: 9
Worksheet time: 5mins
The higher the concentration of gel...
the faster the molecules migrate
the slower the molecules migrate
the larger the pores
What is the function of ammonium persulphate in polyacrylamide gel?
act as catalyst for polymerization
initiator for TEMED catalysis activity
act as crosslinker between acrylamide and bisacrylamide
source of free persulphate radicals
Which is NOT the effect of SDS in PAGE?
disrupts all bonds in protein secondary and tertiary structure
disrupts all noncovalent bonds in protein
denatures protein molecule
adds negative charges to the protein
Which of these is the effect of mercaptoethanol in PAGE?
adds negative charges to the protein
disrupts all bonds in protein secondary and tertiary structure
disrupts disulfide bond in tertiary structure of protein
denatures protein
Why does the agarose gel have to be conducted horizontally?
it migrates protein more efficiently
gravity pull does not play a role in migrating the proteins
it lacks of structural integrity
pore size can be controlled in this orientation
From the video, what is the function of stacking gel?
to stack the proteins together for higher resolution
to gently introduce different pH to the samples
to gently mix the buffers together
to separate the proteins according to pI values
to cause the proteins to be negatively charged
From the video, why does the glycine stopped moving in stacking gel?
it denatured
it had 0 net charge
it was blocked by chloride ions
the gel has small pores
From the video, what made the glycine move again in stacking gel region?
the sandwiching of protein was successful
it had net negative charge again
pushed by the chloride ions
the application of eluting buffer
Why cant we use water as buffer in PAGE?
the hydride ions will migrate the protein molecules in extreme speed
hydrogen ion can shift the pH value
because water has a pH of near neutral
water will evaporate too quickly
