WorksheetsProtoplast: Introduction & Isolation
Total questions: 10
Worksheet time: 5mins
Protoplasts are__________
Cell without a nucleus
Cell without a Cell wall
Cell without a Plasma membrane
Cell without a genetic material
__________ is the first step for protoplast culture
Selection of Explant
Viability testing for protoplast
Isolation of protoplast
Preparation of culture media
Cellulase enzyme in isolation of protoplast is used ________
To degrade proteins
To degrade cellulose
To degrade pectin
To degrade hemicellulose
The production of hybrid plants through fusion of two different somatic protoplasts is known as
Protoplast hybrids
Protoplast fusion
Somatic hybridization
Somatic hybrids
1st report on protoplast fusion-derived somatic hybrid plant was reported by
Carlson et al
Hanstein
Klercker
Cocking
The word “PROTOPLAST” was coined by
Hanstein
Klercker
Cocking
Tkebe et al
Isolation of protoplasts mechanically from higher plants was pioneered by
Klercher
Tkebe et al
Cocking
Both A & B
The principle deficiency of mechanical method of isolation is _________
protoplasts released are few in number.
protoplasts are damaged
Viability of protoplasts is high
All of the above
The most commonly used pectinase is
Macerozyme
Rhozyme HP 150
Cellulase
Pectiolose
Friable tissues are having _________
low starch content generally yields better results
high starch content generally yields better results
low sucrose content generally yields better results
low sucrose content generally yields better results
