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r DNA

Total questions: 7

Worksheet time: 2mins

Name
Class
Date
1.

An enzyme used to lyse proteins

a)

ligase

b)

nuclease

c)

proteinase

d)

liposomes

2.

A double stranded synthetic fragment of DNA with restricted ends are

a)

probe

b)

linker

c)

primer

d)

adaptor

3.

The enzyme that adds poly (A) tail at the 3' end of DNA

a)

TERMINAL TRANSFERESE

b)

T4 poly nucleotide kinase

c)

S1 nuclease

d)

Ligase

4.

Which among the following is not an ideal characteristic of a vector

a)

they should have genetic marker

b)

Origin of replication is present

c)

they should be large in size

d)

No pathogenicity.

5.

Point out the wrong statement about electrophoresis

a)

Its based on the size of the DNA fragments.

b)

Ethidium bromide is used in the running buffer.

c)

DNA fragments move from the negative side to the positive electrode side

d)

glycerol is used in the loading buffer.

6.

Microcarriers are a part of :

a)

liposomes

b)

electroporation

c)

microinjection

d)

biolistic

7.

Vir protein that is bound covalently to the 5' end of ss T DNA is

a)

Vir D2

b)

Vir C1

c)

Vir B1

d)

Vir F