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Basics of chromatography

Total questions: 20

Worksheet time: 12mins

Name
Class
Date
1.
The physical separation of mixtures into individual components is..
a)
chromatography
b)
opacity 
c)
latent
d)
trace evidence 
2.

Ink can be separated by a method of -----------------------------------------------

a)

distillation

b)

filtration

c)

chromatography

d)

sedimentation

3.
A low retention time indicates
a)
a high affinity to the mobile phase
b)
a low affinity to the stationary phase
c)
a high solubility in the mobile phase
d)
all of the above
4.
The substance in the mixture that has the greatest affinity for the stationary phase will travel
a)
fastest
b)
slowest
5.

What is the correct image of paper chromatography???

a)
b)
c)
6.

What is chromatography?

a)

A way to see pretty colors

b)

A laboratory technique used to separate a mixture.

c)

A form of distillation

7.

Which of the following mixture/s is/are separated by chromatography?

a)

Pigments from natural colours

b)

Colours in a dye

c)

Colours of flowers extracts

d)

All the above

8.
Why is Chromatography useful?
a)
It can be used as medicine.
b)
It can be used for finding clues at a crime scene
c)
It can be used to make bombs.
d)
It can reduce the lack of food and water.
9.
Why must the base line be drawn in pencil and not pen?
a)
a) Pencil is good at keeping substances in place.
b)
b) Pencil is soluble in water
c)
c) Pencil is insoluble in water
d)
d) Pencil is not coloured
10.

If a substance is not soluble in the solvent, what happens to the spot placed on the start line?

a)

a) Dot moves to the top of the paper

b)

b) Dot does not move at all

c)

c) Dot moves to the left or right

d)

d) Dot moves down to the bottom of the paper

11.

The stationary phase in this experiment is...

a)

chromatography paper

b)

water

c)

beaker

d)

dye

12.
The mobile phase in paper chromatography is typically a
a)
plasma
b)
solid
c)
gas
d)
liquid
13.
The retention factor is used
a)
to zero the chromatography scale
b)
to identify unknown compounds in a sample
c)
to retain solvent
d)
as a starting tool for chromatography
14.
_________________ utilizes a stationary and a mobile phase to separate mixtures based on their affinity for each phase.
a)
filtration
b)
chromatography
c)
distillation
d)
osmosis
15.

Thin layer chromatography is

a)

partition chromatography

b)

electrical mobility of ionic species

c)

adsorption chromatography

d)

none of the above

16.

Ion-Exchange chromatography:

a)

separate and analyze compounds that can be vaporized without decomposition

b)

Also known as Liquid-Solid Chromatography

c)

Materials used are either cation or anion resins

d)

Separation based on partition coefficient between 2 immiscible liquids

17.

TLC stands for...

a)

Tender Love & Care

b)

Thick Layer Chromatography

c)

Thin Layer Chromatography

d)

Thin Liquid Chromatography

18.

The distance travelled by the compound depends on how soluble it is in the chosen solvent.

a)

TRUE

b)

FALSE

19.

What is normally required to visualise the spots on a TLC chromatogram? Tick all that apply.

a)

X-rays

b)

Ultraviolet light

c)

Infrared light

d)

Developing agent

20.

How to calculate Rf?

a)

Rf = distance travelled by sample / distance travelled by solvent

b)

Rf = distance travelled by solvent / distance travelled by sample