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MICROBIAL IDENTIFICATION SYSTEM

Total questions: 27

Worksheet time: 17mins

Name
Class
Date
1.

ACTIVE ingredient of VP-Voges-Proskauer (acetoin production)

a)

Glucose

b)

Sodium Pyruvate

c)

Arginine

d)

a-Naphthol derivative

e)

Lysine

2.

Active ingredient of N-Nitrate Reductase

a)

Potassium Nitrate

b)

Sulfanilic Acid

c)

a-naphthylamine derivative

d)

acetic anhydride

3.

ACTIVE INGREDIENT OF Phenylalanine deaminase(PD)

a)

Phenylalanine

b)

Ferric chloride

c)

anhydride

4.

ACTIVE INGREDIENT OF Hydrogen sulfide( H2S)

a)

Sodium Thiosulfate

b)

Lead acetate

c)

acetic acetate

5.

ACTIVE INGREDIENT OF Indole(I)

a)

Tryptophane

b)

p-Dimethylaminobenzaldehyde

c)

Hydrochloric acid

6.

ACTIVE INGREDIENT OF Ornithine Decarboxylase (OD)

a)

Ornithine

b)

Bromcresol purple

c)

methylene blue

7.

ACTIVE INGREDIENT OF Lysine Decarboxylase

a)

Lysine

b)

Bromcresol purple

c)

Methylene green

8.

ACTIVE INGREDIENT OF Malonate Utilization

a)

Sodium Malonate

b)

Bromthymol Blue

c)

Methylene blue

9.

ACTIVE INGREDIENT OF UREASE

a)

Urea

b)

arcaria

c)

Cresol red

10.

ACTIVE INGREDIENT OF Esculin Hydrolysis

a)

Esculin

b)

Ferric ammonium citrate

c)

sulfur powder

11.

ACTIVE INGREDIENT OF ONPG B-galactosidase

a)

ONPG

b)

Sorbitol

c)

arabinose

12.

ACTIVE INGREDIENT OF Arabinose Fermentation

a)

arabinose

b)

Bromcresol purple

13.

ACTIVE INGREDIENT OF Adonitol Fermentation

a)

Adonitol

b)

Bromcresol purple

c)

Bromcresol green

14.

ACTIVE INGREDIENT OF Inositol Fermentation (INOS)

a)

Inositol

b)

Bromcresol purple

c)

Bromcresol red

15.

ACTIVE INGREDIENT OF Sorbitol (SORB)

a)

sorbitol

b)

Bromcresol purple

c)

Bromcresol red

16.

VP TEST INTERPRETATION

a)

Positive: Pink to red

negative: Light yellow

b)

Positive: red

negative: Colorless to light pink

c)

Positive: Green

negative: Light yellow

d)

Positive: Brown to black

negative: white

17.

TEST INTERPRETATION Nitrate reductase

a)

Positive: red

negative: Colorless to light pink

b)

Positive: Green

negative: Light yellow

c)

Positive: Brown to black

negative: white

d)

Positive: Pink to red

negative: Light yellow to orange

18.

TEST INTERPRETATION Phenylalanine deaminase

a)

Positive: Green

negative: Light yellow

b)

Positive: Brown to black

negative: white

c)

Positive: Pink to red

negative: Light yellow to orange

19.

TEST INTERPRETATION of H2S

a)

Positive: red

negative: Colorless to light pink

b)

Positive: Brown to black

negative: white

c)

Positive: Pink to red

negative: Light yellow

20.

TEST INTERPRETATION Indole

a)

Positive: Pink to red

negative: Light yellow to orange

b)

Positive: Green

negative: Light yellow

c)

Positive: red

negative: Colorless to light pink

21.

TEST INTERPRETATION of Ornithine decarboxylase

a)

Positive: Purple to red purple

negative: Amber to yellow

b)

Positive: Brown to black

negative: white

22.

TEST INTERPRETATION Lysine Decarboxylase

a)

Positive: Purple to red purple

negative: Amber to yellow

b)

Positive: Brown to black

negative: white

c)

Positive: red

negative: Colorless to light pink

23.

TEST INTERPRETATION Malonate utilization

a)

Positive: green to blue

negative: yellow

b)

Positive: Orange to red purple

negative: yellow

24.

TEST INTERPRETATION of Urease

a)

Positive: Orange to red purple

negative: yellow

b)

Positive: Yellow to amber

negative: Red purple to purple

25.

TEST INTERPRETATION of Esculin Hydrolysis

a)

Positive: Brown to Black

negative: No color change/Beige

b)

Positive: Yellow to amber

negative: Red purple to purple

26.

TEST INTERPRETATION of ARAB, ADON, INOS, SORB

a)

Positive: Yellow to amber

negative: Red purple to purple

b)

Positive: Brown to Black

negative: No color change/Beige

27.

TEST INTERPRETATION of ONPG

a)

Positive: light yellow to yellow

negative: colorless

b)

Positive: Orange to red purple

negative: yellow