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WorksheetsPAGE Gel Electrophoresis
Total questions: 14
Worksheet time: 9mins
Which of the following steps of PAGE electrophoresis would come FIRST?
Pipette protein samples into wells
Remove cassette from gel
Immerse gel into stain
Place casette in gel box at the correct orientation
SDS- PAGE Gel Electrophoresis is used to determine the molecular weight of (a) .
Which of the following is a way that a PAGE gel is different than an agarose gel?
PAGE gels are made of polyacrylamide
PAGE gels often have a gradient of gel concentrations
PAGE gels require the use of TBE buffer
PAGE requires the use of SDS buffer
What is the function of SDS buffer in vertical electrophoresis?
to track the passage of the sample
it will ensure the proteins are denatured
a reducing agent
to increase the density of the sample
When placing a PAGE gel cassette in a gel box, the tall side of the cassette should be oriented towards...
The side of the box with the gel cassette clip
The middle of the gel box
Before protein samples can be loaded into wells, what needs to happen first?
All buffer must be removed from wells
The gel box needs to be turned on
The protein samples need to be denatured in the water bath
The protein samples need to be placed in an ice bath for 30 minutes
Proteins that are in their normal biologically active form are called...
(a)
Where can you find the positive electrode in a vertical gel electrophoresis box?
At the top
At the bottom
On the side
Which of the following is NOT true of the gels used in PAGE electrophoresis?
They have a concentration of 0.8%
They allow the smallest proteins to migrate the farthest
They must be purchased premade
They are very thin and are therefore stored in a plastic cassette
In order to accurately estimate protein molecular weight using PAGE, the proteins must be...
Isoelectric
Native
Denatured
Positive
If you forgot to denature your proteins, which kind of protein would migrate through a PAGE gel the fastest?
Rod shaped
Spiral shaped
Elliptical shaped
Sphere shaped
What unit is used for protein molecular weight?
(a)
What is the purpose of the loading buffer in PAGE electrophoresis?
To provide color and density to the sample
To denature the proteins
To act as a running buffer
To stain the proteins
What is the effect of increasing the concentration of the gel in PAGE electrophoresis?
It increases the migration speed of proteins
It decreases the migration speed of proteins
It has no effect on the migration speed of proteins
It denatures the proteins
