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Molecular Biology Quiz Buster 1

Total questions: 23

Worksheet time: 46mins

Name
Class
Date
1.

Catabolite repression of the Lac operon uses what molecules as Inducer and activator?

a)

a. Glucose and Lactose

b)

b. ATP and cAMP

c)

c. cAMP and CAP

d)

d. CAP and Adenylcyclase

2.

What is the likely mutation if structural genes of the Lac operon are made in the presence of high Glucose concentration (select all correct answers)?

a)

a. Z-

b)

b. Y-

c)

c. OC

d)

d. Pi-

3.

RNA Polymerase and Sigma Factor form what?

a)

a. Basal transcription apparatus

b)

b. Operon

c)

c. Enhancer

d)

d. Holoenzyme

4.

Which of the following is a stop codon?

a)

a. GAT

b)

b. TGA

c)

c. AUG

d)

d. TTA

5.

An Expression Vector differs from a cloning vector in that it contains:

a)

a. Origin of Replication

b)

b. Selectable Markers

c)

c. Multiple Cloning Site

d)

d. Genetic Elements for Transcription/Translation

6.

The mediator is part of the Basal Transcription Apparatus.

a)

True

b)

False

7.

An element is transcribed.

a)

True

b)

False

8.

Heat and light are potential stimuli in the activation of response elements.

a)

True

b)

False

9.

Hox genes are only seen in reptiles and insects such as drosophila.

a)

True

b)

False

10.

A Temperate phage is one that can enter either Lysogenic or Lytic pathways.

a)

True

b)

False

11.

Both qRT-PCR and RNA-seq measure RNA by first converting to cDNA for amplification.

a)

True

b)

False

12.

pET on/off and tet on/off describes two states of the same system.

a)

True

b)

False

13.

In remodelling of Chromatin:

a)

a. Histones are not altered chemically

b)

b. Promotors or Transcription Factor binding sites are revealed to allow transcription

c)

c. ATP dependent

d)

d. All of the Above

14.

A regulatory promotor (select all correct answers):

a)

a. Can be affected by multiple TFs

b)

b. Regulates transcription of structural genes

c)

c. Contains different combinations of consensus sequences allowing multiple basal transcription apparatuses to bind

d)

d. Contains the starting point of transcription

15.

A signalling molecule that does not enter a cell but can induce a signal cascade to affect transcription within it is most likely a:

a)

a. Steroid

b)

b. Peptide

c)

c. Complex

d)

d. Nucleic Acid

16.

mRNA Stability is influenced by which of the following (select all correct answers)?

a)

a. poly(A) tail

b)

b. 3’ AU rich UTR

c)

c. Hormones

d)

d. siRNA or miRNA

17.

What statement is correct?

a)

a. Dicer cleaves single stranded RNA

b)

b. Drosha cleaves mRNA as part of the RISC complex

c)

c. Argonaut cleaves Pri-miRNA in the nucleus

d)

d. RISC is formed by ssRNA and an RNP Granule

18.

Where is primary miRNA cleaved?

a)

a. Nucleus

b)

b. cytoplasm

c)

c. Endoplasmic Reticulum

d)

d. Pri-miRNA is not cleaved

19.

Which of the following techniques is used to detect the presence of a protein?

a)

a. Southern Blot

b)

b. Western Blot

c)

c. RT-qPCR

d)

d. Agarose Gel Electrophoresis

20.

Proteins are denatured and negatively charged by what chemical?

a)

a. Coomassie Blue

b)

b. SDS

c)

c. Horse Radish Peroxidase

d)

d. Midori green

21.

2D gels separate proteins based on what?

a)

a. Size

b)

b. Isoelectric Point

c)

c. Tertiary Structure

d)

d. both a and b

22.

A particular human gene is unmethylated in ovaries but methylated in testicles. In the following family tree, parents A & 1 produce two offspring: 2 who marries C and B who marries 3. C & 2 have offspring D while B & 3 have offspring 4.


Of the children D and 4, who is transcribing grandmother A's allele?

a)

D

b)

4

23.

Protein measuring methods involve the following except for?

a)

a. Western Blot

b)

b. 2D Electrophoresis

c)

c. Proteomics

d)

d. Southern Blot