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WorksheetsMolecular Biology Quiz Buster 1
Total questions: 23
Worksheet time: 46mins
Catabolite repression of the Lac operon uses what molecules as Inducer and activator?
a. Glucose and Lactose
b. ATP and cAMP
c. cAMP and CAP
d. CAP and Adenylcyclase
What is the likely mutation if structural genes of the Lac operon are made in the presence of high Glucose concentration (select all correct answers)?
a. Z-
b. Y-
c. OC
d. Pi-
RNA Polymerase and Sigma Factor form what?
a. Basal transcription apparatus
b. Operon
c. Enhancer
d. Holoenzyme
Which of the following is a stop codon?
a. GAT
b. TGA
c. AUG
d. TTA
An Expression Vector differs from a cloning vector in that it contains:
a. Origin of Replication
b. Selectable Markers
c. Multiple Cloning Site
d. Genetic Elements for Transcription/Translation
The mediator is part of the Basal Transcription Apparatus.
True
False
An element is transcribed.
True
False
Heat and light are potential stimuli in the activation of response elements.
True
False
Hox genes are only seen in reptiles and insects such as drosophila.
True
False
A Temperate phage is one that can enter either Lysogenic or Lytic pathways.
True
False
Both qRT-PCR and RNA-seq measure RNA by first converting to cDNA for amplification.
True
False
pET on/off and tet on/off describes two states of the same system.
True
False
In remodelling of Chromatin:
a. Histones are not altered chemically
b. Promotors or Transcription Factor binding sites are revealed to allow transcription
c. ATP dependent
d. All of the Above
A regulatory promotor (select all correct answers):
a. Can be affected by multiple TFs
b. Regulates transcription of structural genes
c. Contains different combinations of consensus sequences allowing multiple basal transcription apparatuses to bind
d. Contains the starting point of transcription
A signalling molecule that does not enter a cell but can induce a signal cascade to affect transcription within it is most likely a:
a. Steroid
b. Peptide
c. Complex
d. Nucleic Acid
mRNA Stability is influenced by which of the following (select all correct answers)?
a. poly(A) tail
b. 3’ AU rich UTR
c. Hormones
d. siRNA or miRNA
What statement is correct?
a. Dicer cleaves single stranded RNA
b. Drosha cleaves mRNA as part of the RISC complex
c. Argonaut cleaves Pri-miRNA in the nucleus
d. RISC is formed by ssRNA and an RNP Granule
Where is primary miRNA cleaved?
a. Nucleus
b. cytoplasm
c. Endoplasmic Reticulum
d. Pri-miRNA is not cleaved
Which of the following techniques is used to detect the presence of a protein?
a. Southern Blot
b. Western Blot
c. RT-qPCR
d. Agarose Gel Electrophoresis
Proteins are denatured and negatively charged by what chemical?
a. Coomassie Blue
b. SDS
c. Horse Radish Peroxidase
d. Midori green
2D gels separate proteins based on what?
a. Size
b. Isoelectric Point
c. Tertiary Structure
d. both a and b
A particular human gene is unmethylated in ovaries but methylated in testicles. In the following family tree, parents A & 1 produce two offspring: 2 who marries C and B who marries 3. C & 2 have offspring D while B & 3 have offspring 4.
Of the children D and 4, who is transcribing grandmother A's allele?
D
4
Protein measuring methods involve the following except for?
a. Western Blot
b. 2D Electrophoresis
c. Proteomics
d. Southern Blot
